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Invitrogen
Rat anti-Mouse IgG1 (Heavy chain) Secondary Antibody, FITC
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Mouse IgG1 (Heavy chain) Secondary Antibody (MA5-16785) in ICC/IF
Immunofluorescence analysis of Rat anti-Mouse IgG1 (Heavy chain) Secondary Antibody, FITC conjugate was performed using HeLa cells stained with alpha Tubulin (23610501) Mouse Monoclonal Primary Antibody (Product # A11126). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with Mouse primary antibody ... View More
产品信息
MA5-16785
应用
建议稀释比
免疫细胞化学 (ICC/IF)
1 µg/mL
流式细胞分析 (Flow)
5 µg/mL
产品规格
种属反应
Mouse
宿主/亚型
Rat
/ IgG1
分类
Monoclonal
类型
Secondary Antibody
克隆号
LO-MG1-2
抗原
Purified murine IgG1 from BALB/c mice
偶联物
激发/发射光谱
498/517 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS with 50% glycerol
内含物
<0.1% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2538268
靶标
IgG1 (Heavy chain)
抗体形式
Whole Antibody
产品详细信息
For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Rat anti Mouse IgG1 Heavy Chain antibody, clone LO-MG1-2 recognizes murine IgG1, and does not bind other mouse immunoglobulin classes or subclasses.
靶标信息
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
