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Mouse IgG1 Secondary Antibody (18-4015-80) in WB
Chemiluminescent western blot was performed using Rat anti-Mouse IgG1 Secondary Antibody, HRP, eBioscience™ (Product # 18-4015-80). Whole cell extracts of A-431 (Lane 1, 2, 3), HaCaT (Lane 4, 5, 6), SH-SY5Y (Lane 7), HeLa (Lane 8) and MCF7 (Lane 9) were electrophoresed usingNuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were transferred onto anitrocellulose membrane (Product # I... View More
产品信息
18-4015-80
应用
建议稀释比
免疫印迹 (WB)
1:500-1:5,000
免疫组化（石蜡） (IHC (P))
20 µg/mL
免疫细胞化学 (ICC/IF)
20 µg/mL
酶联免疫吸附实验 (ELISA)
Assay-Dependent
产品规格
种属反应
Mouse
宿主/亚型
Rat
/ IgG
分类
Monoclonal
类型
Secondary Antibody
克隆号
M1-14D12
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
no preservative
保存条件
4° C
运输条件
Wet ice
RRID
AB_11040787
靶标
IgG1
抗体形式
Whole Antibody
产品详细信息
Description: The monoclonal antibody M1-14D12 recognizes Mouse IgG1 antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other mouse isotypes nor does it crossreact to rat IgG1 or any rat isotype antibodies.
Applications Reported: This M1-14D12 antibody has been reported for use in western blotting, ELISA, immunohistochemical and immunocytochemical staining.
Applications Tested: This M1-14D12 antibody has been tested by immunocytochemistry and immunohistochemistry after staining with a primary antibody of the mouse IgG1 isotype. This can be used at less than or equal to 20 µg/mL. For western blotting, this can be used at 1 µg/mL (1/500 dilution). It is recommended that the antibody be titrated for optimal performance in the assay of interest.
靶标信息
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
