Invitrogen

Rb (Cleaved DRb-p70) Monoclonal Antibody (172C1094)

抗体检测数据 (1)

Rb (Cleaved DRb-p70) Antibody in Western Blot (WB)

Rb (Cleaved DRb-p70) Antibody in Western Blot (WB)

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Rb (Cleaved DRb-p70) Antibody (MA1-41186) in WB

Western blot analysis of Rb (Cleaved DRb-p70) in HL60 cells. Samples were incubated in Rb (Cleaved DRb-p70) monoclonal antibody (Product # MA1-41186). Treated with camptothecin for 24 hours.

Rb (Cleaved DRb-p70) Antibody in Western Blot (WB)

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Rb (Cleaved DRb-p70) Antibody in Western Blot (WB) — Western blot analysis of Rb (Cleaved DRb-p70) in HL60 cells. Samples were incubated in Rb (Cleaved DRb-p70) monoclonal antibody (Product # MA1-41186). Treated with camptothecin for 24 hours.

产品信息

MA1-41186

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2 µg/mL

-

免疫组化（石蜡） (IHC (P))

Assay-Dependent

-

免疫组化（冰冻） (IHC (F))

1:10-1:500

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

172C1094

抗原

Caspase-3-induced cleavage site of human Rb.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein G

保存液

PBS with 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_1075256

产品详细信息

Suggested positive control: camptothecin-treated HL-60 (24 hr).

靶标信息

The retinoblastoma gene product (Rb) is a tumor suppressor protein and acts as cell cycle regulator (1). Recently, Rb has been shown to play a role in apoptosis. During apoptosis, Rb is subjected to cleavage by caspase-3 like protease. Caspase-3 cleaves Rb into 48 and 68kDa fragments (2-4). Cleavage of Rb would make it functionally inactive, causing release of E2F-1. The E2F-1 then may promote apoptosis (5).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Cleaved Rb; delta RB-p70; DRb-p70; exon 17 tumor GOS561 substitution mutation causes premature stop; GOS563 exon 17 substitution mutation causes premature stop; p105-Rb; p110-RB1; pp105; pp110; pRb; prepro-retinoblastoma-associated protein; protein phosphatase 1, regulatory subunit 130; Rb; Rb-p70; retinoblastoma 1; retinoblastoma suspectibility protein; Retinoblastoma-associated protein

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基因别名: OSRC; p105-Rb; pp110; PPP1R130; pRb; RB; Rb-1; RB1

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UniProt ID: (Human) P06400, (Mouse) P13405

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Entrez Gene ID: (Human) 5925, (Mouse) 19645

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core promoter binding RNA polymerase II activating transcription factor binding DNA binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity protein binding transcription factor binding kinase binding ubiquitin protein ligase binding identical protein binding androgen receptor binding phosphoprotein binding enzyme binding chromatin/chromatin-binding, or -regulatory protein

参与通路

cell cycle checkpoint G1/S transition of mitotic cell cycle regulation of transcription involved in G1/S transition of mitotic cell cycle chromatin remodeling transcription, DNA-templated negative regulation of protein kinase activity cell cycle arrest negative regulation of transcription from RNA polymerase II promoter during mitosis mitotic cell cycle checkpoint Ras protein signal transduction regulation of mitotic cell cycle negative regulation of gene expression viral process covalent chromatin modification androgen receptor signaling pathway sister chromatid biorientation neuron projection development maintenance of mitotic sister chromatid cohesion glial cell apoptotic process skeletal muscle cell differentiation neuron maturation enucleate erythrocyte differentiation negative regulation of sequence-specific DNA binding transcription factor activity regulation of lipid kinase activity myoblast differentiation positive regulation of macrophage differentiation positive regulation of mitotic metaphase/anaphase transition negative regulation of smoothened signaling pathway negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter digestive tract development cell morphogenesis involved in neuron differentiation negative regulation of epithelial cell proliferation striated muscle cell differentiation cell division neuron apoptotic process protein localization to chromosome, centromeric region cellular response to xenobiotic stimulus regulation of cohesin loading negative regulation of transcription involved in G1/S transition of mitotic cell cycle regulation of centromere complex assembly hepatocyte apoptotic process negative regulation of G1/S transition of mitotic cell cycle positive regulation of transcription regulatory region DNA binding negative regulation of transcription from RNA polymerase II promoter regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter cell cycle negative regulation of cell proliferation chromatin modification neuron differentiation negative regulation of cell cycle negative regulation of mitotic cell cycle regulation of cell cycle negative regulation of hepatocyte apoptotic process