Invitrogen

RhoA Recombinant Rabbit Monoclonal Antibody (SN0612)

RhoA Antibody in Immunocytochemistry (ICC/IF)

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RhoA Antibody (MA5-32262) in ICC/IF

Immunofluorescence analysis of RhoA was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with RhoA Recombinant Rabbit Monoclonal Antibody (SN0612) (Product # MA5-32262) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluo... View More

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产品信息

MA5-32262

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:2,000

免疫细胞化学 (ICC/IF)

1:50-1:200

流式细胞分析 (Flow)

1:50-1:100

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

SN0612

抗原

Synthetic peptide within Human aa 144-193

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2809548

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

RhoA regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. It is involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis, and plays an essential role in cleavage furrow formation. RhoA is required for the apical junction formation of keratinocyte cell-cell adhesion, and serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. RhoA stimulates PKN2 kinase activity and may be an activator of PLCE1. It is activated by ARHGEF2, which promotes the exchange of GDP for GTP. It is essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Aplysia ras-related homolog 12; aplysia ras-related homolog A; aplysia ras-related homolog A1; aplysia ras-related homolog A2; h12; MGC72339; oncogene RHO H12; plysia ras-related homolog A2; Ras family member A; ras homolog A1; ras homolog A2; ras homolog gene family, member A; ras homolog gene family, member A1; ras homolog gene family, member A2; Rho; Rho cDNA clone 12; Rho family GTPase; small GTP binding protein RhoA; Transforming protein RhoA

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基因别名: ARH12; ARHA; Arha1; Arha2; RHO12; RHOA; RHOH12

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UniProt ID: (Human) P61586, (Rat) P61589, (Mouse) Q9QUI0

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Entrez Gene ID: (Human) 387, (Rat) 117273, (Mouse) 11848

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功能

GTPase activity protein binding GTP binding myosin binding GDP binding protein domain specific binding Rho GDP-dissociation inhibitor binding nucleotide binding small GTPase

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