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Rhodopsin Antibody (MA5-47925) in WB
Western Blot analysis of Rhodopsin was performed using Rhodopsin Recombinant Monoclonal Antibody (Product # MA5-47925). Rhodopsin expressing plasmid transfected (A) and non-transfected (B) HEK293 cells lysate (3 µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots probed with Rhodopsin Recombinant Monoclonal Antibody at 0.1 µg/mL before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
产品信息
MA5-47925
应用
建议稀释比
已发表文章
产品规格
种属反应
Amphibian,
Bovine,
Human,
Mouse,
Rat,
Zebrafish
宿主/亚型
Rabbit
/ IgG, kappa
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
Rho 1D4
抗原
Bleached bovine rod outer segment (ROS).
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
0.02% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_3074369
产品详细信息
Specificity: The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes. The epitope has the following amino acid sequence: 'TETSQVAPA'. A limitation of the 1D4 tag, however, is that it has to be placed at the C-terminus of a protein. This is because the Rho1D4 monoclonal antibody requires a free carboxylate group for high affinity binding. Amidation of the carboxyl group lowers its immunoreactivity to the Rho1D4 antibody by over 100 fold.
靶标信息
Visual pigments such as rhodopsin and porphyropsin are light-absorbing molecules that mediate vision. Rhodopsin consists of an apoprotein, opsin, covalently linked to 11-cis-retinal. This receptor is coupled to the activation of phospholipase C. Porphyropsin consists of opsin covalently linked to 11-cis 3,4-didehydroretinal.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: L opsin; Long Wavelength Sensitive opsin; LWS opsin; MGC138309; MGC138311; OPN 2; opsin 2; opsin 2, rod pigment; Opsin-2; Red Opsin; retinal rod opsin pigment rh1.1; Rhodopsin; rhodopsin (opsin 2, rod pigment) (retinitis pigmentosa 4, autosomal dominant); Rhodopsin (retinitis pigmentosa 4, autosomal dominant); rhodopsin-like-1.1 rod opsin; Rod Opsin; RP 4; seven transmembrane light receptor
基因别名: CSNBAD1; Noerg1; OPN2; Ops; Rh; RH1; rh1.1; RHO; RP4; wu:fi06d11; zfo2; zfrho
UniProt ID: (Bovine) P02699, (Human) P08100, (Rat) P51489, (Mouse) P15409, (Zebrafish) P35359
Entrez Gene ID: (Bovine) 509933, (Human) 6010, (Rat) 24717, (Mouse) 212541, (Zebrafish) 30295
G-protein alpha-subunit binding
opsin binding
guanyl-nucleotide exchange factor activity
11-cis retinal binding
protein binding
G-protein coupled photoreceptor activity
zinc ion binding
identical protein binding
arrestin family protein binding
G-protein coupled receptor activity
retinal binding
metal ion binding
photoreceptor activity
spectrin binding
signal transducer activity
G-protein coupled receptor
protein phosphorylation
visual perception
phototransduction, visible light
response to light stimulus
absorption of visible light
rhodopsin mediated signaling pathway
protein-chromophore linkage
photoreceptor cell maintenance
retina development in camera-type eye
retinoid metabolic process
G-protein coupled receptor signaling pathway
red, far-red light phototransduction
regulation of rhodopsin mediated signaling pathway
phototransduction
detection of light stimulus
sensory perception of light stimulus
cellular response to light stimulus
signal transduction
response to stimulus
