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Application
FIGURE: 1 / 3
Ryanodine Receptor 2 Antibody (ARR-002-200UL) in IHC (P)
Expression of RyR2 in rat cardiac muscle - Immunohistochemical staining of paraffin-embedded sections of ratmyocardium using Anti-Ryanodine Receptor 2 Antibody (#ARR-002), (1:50). Staining is specific for cardiomyocytes while smooth muscles cells in the artery walls are negative (red arrows). Hematoxilin is used as the counterstain.
Product Details
ARR-002-200UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
CAGESMSPGQGRNN, corresponding to amino acid residues 1489-1502 of human RyR2, Intracellular, N-terminus
Conjugate
Unconjugated
Form
Lyophilized
Concentration
0.8 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
Dihydropyridine receptor (DHPR) is a surface membrane protein critical for the excitation-contraction coupling of striated muscle. DHPR and the sarcoplasmic reticulum ryanodine receptor (RyR) are two key components of the intracellular junctions, where depolarization of the surface membrane is converted into the release of Ca2+ from internal stores. The alpha1-subunit of the DHPR contains a cytoplasmic loop which is thought to be involved in the interactions with RyR. Phosphorylation of the DHPR alpha1-subunit is also thought to play a role in the functional interaction of DHPR and RyR. Mutation in DHPR alpha1 results in excitation-contraction uncoupling, leading to muscular dysgenesis, a complete inactivity in developing skeletal muscles. Cells that do not express RyR also lack excitation-contraction coupling and exhibit a severalfold reduction in Ca2+ current density.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: calcium release channel; Cardiac muscle ryanodine receptor; Cardiac muscle ryanodine receptor-calcium release channel; cardiac-type ryanodine receptor; hRYR-2; islet-type ryanodine receptor; kidney-type ryanodine receptor; Ryanodine receptor; Ryanodine receptor 2; ryanodine receptor 2 (cardiac); ryanodine receptor type 2; ryanodine receptor type II; RYR-2; Type 2 ryanodine receptor
Gene Aliases: 9330127I20Rik; ARVC2; ARVD2; RyR; RYR-2; RYR2; VTSIP
UniProt ID: (Human) Q92736, (Rat) B0LPN4, (Mouse) E9Q401
Entrez Gene ID: (Human) 6262, (Rat) 689560, (Mouse) 20191
ryanodine-sensitive calcium-release channel activity
calcium channel activity
calcium ion binding
protein binding
calmodulin binding
calcium-release channel activity
enzyme binding
protein kinase A catalytic subunit binding
protein kinase A regulatory subunit binding
identical protein binding
protein self-association
suramin binding
ion channel binding
calcium-induced calcium release activity
protein kinase binding
scaffold protein binding
organic cyclic compound binding
ion channel activity
ligand-gated ion channel
scaffold/adaptor protein
response to hypoxia
regulation of heart rate
embryonic heart tube morphogenesis
left ventricular cardiac muscle tissue morphogenesis
cardiac muscle hypertrophy
detection of calcium ion
calcium ion transport
cellular calcium ion homeostasis
positive regulation of heart rate
regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
regulation of cardiac muscle contraction by calcium ion signaling
release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
response to muscle activity
calcium-mediated signaling
BMP signaling pathway
response to caffeine
ion transmembrane transport
calcium-mediated signaling using intracellular calcium source
response to muscle stretch
release of sequestered calcium ion into cytosol
positive regulation of sequestering of calcium ion
regulation of cytosolic calcium ion concentration
response to redox state
regulation of cardiac muscle contraction
cardiac muscle contraction
canonical Wnt signaling pathway
calcium ion transport into cytosol
sarcoplasmic reticulum calcium ion transport
cellular response to caffeine
cellular response to epinephrine stimulus
establishment of protein localization to endoplasmic reticulum
ventricular cardiac muscle cell action potential
Purkinje myocyte to ventricular cardiac muscle cell signaling
cell communication by electrical coupling involved in cardiac conduction
type B pancreatic cell apoptotic process
positive regulation of the force of heart contraction
regulation of AV node cell action potential
regulation of SA node cell action potential
regulation of atrial cardiac muscle cell action potential
regulation of ventricular cardiac muscle cell action potential
positive regulation of calcium-transporting ATPase activity
regulation of cardiac conduction
response to nutrient
response to magnesium ion
response to drug
negative regulation of cytosolic calcium ion concentration
response to calcium ion
cytosolic calcium ion transport
calcium ion transmembrane transport
manganese ion transmembrane transport
positive regulation of ryanodine-sensitive calcium-release channel activity by adrenergic receptor signaling pathway involved in positive regulation of cardiac muscle contraction
transport
ion transport
multicellular organism development
transmembrane transport
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