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S100A1 Antibody (PA1-932) in ICC/IF
Immunofluorescent analysis of S100 alpha (red) in HEK293T cells. Cells fixed with 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were probed with a S100 alpha polyclonal antibody (Product # PA1-932) at a dilution of 1:100 overnight at 4°C in 1X PBS containing 1% BSA and 0.3% Triton X-100, washed with 1X PBS, and incubated with a fluorophore-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:200 for 1 hour at room temperature. Nuclei... View More
产品信息
PA1-932
应用
建议稀释比
已发表文章
产品规格
种属反应
Bovine,
Human,
Mouse,
Pig,
Rat
已发表种属
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Purified S100-alpha protein from human pectoral muscle cells.
偶联物
Unconjugated
形式
Lyophilized
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 20mg/mL BSA
内含物
0.1% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2183210
产品详细信息
PA1-932 detects S100-alpha protein in bovine, human, mouse, porcine, and rat samples. This antibody has shown no cross-reactivity with the S100-beta protein.
PA1-932 has successfully been used in Western blot, ELISA, immunofluorescence, immunocytochemistry, immunoprecipitation and immunohistochemical procedures. By Western blot, this antibody detects a 10 kDa protein representing S100-alpha from human muscle. Immunohistochemical staining on paraffin sections demonstrates that S100-alpha is localized in serous cells, myoepithelial cells and epithelial cells of intercalated ducts in the parotid gland after staining with PA1-932.
S100 protein is relatively small and, therefore, it is recommended that the electrophoresis be performed using tricine-SDS-PAGE gels and transferred to a nylon membrane.
The PA1-932 immunizing protein corresponds to purified S100-alpha protein from human pectoral muscle cells.
Reconstitute in 100 µL PBS to create a stock of 1 mg/mL.
靶标信息
S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. S100 protein is also expressed in the antigen presenting cells such as the Langerhans cells in skin and interdigitating reticulum cells in the paracortex of lymph nodes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Protein S100-A1; S-100 protein alpha chain; S-100 protein subunit alpha; S100 alpha; S100 calcium-binding protein A1; S100 protein, alpha polypeptide
基因别名: AI266795; S100; S100-alpha; S100A; S100A1
UniProt ID: (Bovine) P02639, (Human) P23297, (Rat) P35467, (Mouse) P56565
Entrez Gene ID: (Bovine) 528735, (Pig) 106504218, (Human) 6271, (Rat) 295214, (Mouse) 20193
