Invitrogen

S1P1 Polyclonal Antibody

S1P1 Antibody in Immunocytochemistry (ICC/IF)

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S1P1 Antibody (PA1-1040) in ICC/IF

Immunofluorescence analysis of EDG1 was performed using 70% confluent log phase Neuro-2a cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with EDG1 Rabbit Polyclonal Antibody (Product # PA1-1040) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product #... View More

S1P1 Antibody in Immunohistochemistry (Frozen) (IHC (F))

S1P1 Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)

S1P1 Antibody in Immunohistochemistry (IHC)

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产品信息

PA1-1040

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2-4 µg/mL

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免疫组化 (IHC)

Assay-dependent

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免疫组化（冰冻） (IHC (F))

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免疫细胞化学 (ICC/IF)

2 µg/mL

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流式细胞分析 (Flow)

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免疫沉淀 (IP)

Assay-dependent

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to residues S(359) H P Q K D D G D N P E T I(372) of mouse S1P1.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2184729

产品详细信息

PA1-1040 detects S1P1 protein in transfected human and mouse cell samples. This antibody shows no cross-reactivity to S1P3.

PA1-1040 has successfully been used in immuno-fluorescence, immunohistochemistry, immunoprecipitation and Western blot procedures. By Western blot, this antibody detects an ~44 kDa protein representing recombinant S1P1 from transfected HEK293 cells. Immunofluorescence data demonstrates that S1P1 is localized to the plasma membrane after staining with PA1-1040.

The PA1-1040 immunogen is a synthetic peptide corresponding to residues S(359) H P Q K D D G D N P E T I(372) of mouse S1P1. This sequence is completely conserved in rat and 92% conserved in human S1P1. This peptide (Cat. # PEP-220) is available for use in neutralization and control experiments.

靶标信息

S1P1 is a G-protein coupled receptor for the bioactive lysosphingolipid sphingosine 1-phosphate (S1P) that is coupled to the G(i) subclass of heteromeric G proteins. S1P1 signaling leads to the activation of RAC1, SRC, PTK2/FAK1 and MAP kinases. It plays an important role in cell migration via its role in the reorganization of the actin cytoskeleton and formation of lamellipodia in response to stimuli that increase the activity of the sphingosine kinase SPHK1. S1P1 is required for normal chemotaxis toward sphingosine 1-phosphate, normal embryonic heart development, and normal cardiac morphogenesis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Dysregulation of sphingosine-1-phosphate (S1P) and S1P receptor 1 signaling in the 5xFAD mouse model of Alzheimer's disease.

Brain research

Jung Y,Lopez-Benitez J,Tognoni CM,Carreras I,Dedeoglu A

PA1-1040 was used in Western Blotting to reveal that dysregulation of S1P and S1PR signaling may associate with the development of AD-like pathology through Akt/mTor/Tau signaling.

Sun Jan 15 00:00:00 EST 2023

Novel delivery of cellular therapy to reduce ischemia reperfusion injury in kidney transplantation.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Thompson ER,Bates L,Ibrahim IK,Sewpaul A,Stenberg B,McNeill A,Figueiredo R,Girdlestone T,Wilkins GC,Wang L,Tingle SJ,Scott WE,de Paula Lemos H,Mellor AL,Roobrouck VD,Ting AE,Hosgood SA,Nicholson ML,Fisher AJ,Ali S,Sheerin NS,Wilson CH

PA1-1040 was used in Immunocytochemistry to report the first successful delivery of cellular therapy to a human kidney during NMP.

Thu Apr 01 00:00:00 EDT 2021

Novel delivery of cellular therapy to reduce ischemia reperfusion injury in kidney transplantation.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

PA1-1040 was used in Immunocytochemistry to report the first successful delivery of cellular therapy to a human kidney during NMP.

Thu Apr 01 00:00:00 EDT 2021

Inhibition of Sphingosine-1-Phosphate Receptor 2 by JTE013 Promoted Osteogenesis by Increasing Vesicle Trafficking, Wnt/Ca²⁺, and BMP/Smad Signaling.

International journal of molecular sciences

Lin S,Pandruvada S,Yu H

PA1-1040 was used in Western Blot to supported that enhanced osteogenesis in JTE013-treated BMSCs is associated with increased vesicle trafficking, which promotes the synthesis and transport of osteogenic protein and matrix vesicles and enhances matrix mineralization.

Mon Nov 08 00:00:00 EST 2021

Inhibition of Sphingosine-1-Phosphate Receptor 2 by JTE013 Promoted Osteogenesis by Increasing Vesicle Trafficking, Wnt/Ca²⁺, and BMP/Smad Signaling.

International journal of molecular sciences

Lin S,Pandruvada S,Yu H

Mon Nov 08 00:00:00 EST 2021

Silencing of Sphingosine kinase 1 Affects Maturation Pathways in Mouse Neonatal Cardiomyocytes.

International journal of molecular sciences

Jozefczuk E,Szczepaniak P,Guzik TJ,Siedlinski M

PA1-1040 was used in Western Blotting to demonstrate that the Sphk1 silencing in neonatal mouse CMs facilitated their postnatal maturation in both physiological and stress conditions.

Wed Mar 31 00:00:00 EDT 2021

Silencing of Sphingosine kinase 1 Affects Maturation Pathways in Mouse Neonatal Cardiomyocytes.

International journal of molecular sciences

Jozefczuk E,Szczepaniak P,Guzik TJ,Siedlinski M

PA1-1040 was used in Western Blotting to demonstrate that the Sphk1 silencing in neonatal mouse CMs facilitated their postnatal maturation in both physiological and stress conditions.

Wed Mar 31 00:00:00 EDT 2021

Dysregulation of sphingolipid metabolism contributes to bortezomib-induced neuropathic pain.

The Journal of experimental medicine

Stockstill K,Doyle TM,Yan X,Chen Z,Janes K,Little JW,Braden K,Lauro F,Giancotti LA,Harada CM,Yadav R,Xiao WH,Lionberger JM,Neumann WL,Bennett GJ,Weng HR,Spiegel S,Salvemini D

PA1-1040 was used in Western Blotting to study the mechanism by which bortezomib-induced painful peripheral neuropathy develops.

Mon May 07 00:00:00 EDT 2018

Sphingosine-1-Phosphate Receptor 1 Is Involved in Non-Obese Diabetic Mouse Thymocyte Migration Disorders.

International journal of molecular sciences

Lemos JP,Smaniotto S,Messias CV,Moreira OC,Cotta-de-Almeida V,Dardenne M,Savino W,Mendes-da-Cruz DA

Published figure using S1P1 polyclonal antibody (Product # PA1-1040) in Immunofluorescence

Sat May 12 00:00:00 EDT 2018

Sphingosine-1-Phosphate Receptor 1 Is Involved in Non-Obese Diabetic Mouse Thymocyte Migration Disorders.

International journal of molecular sciences

Lemos JP,Smaniotto S,Messias CV,Moreira OC,Cotta-de-Almeida V,Dardenne M,Savino W,Mendes-da-Cruz DA

Published figure using S1P1 polyclonal antibody (Product # PA1-1040) in Immunofluorescence

Sat May 12 00:00:00 EDT 2018

Sphingosine 1-Phosphate Receptor 1 Modulates CNTF-Induced Axonal Growth and Neuroprotection in the Mouse Visual System.

Neural plasticity

Joly S,Dalkara D,Pernet V

PA1-1040 was used in Immunohistochemistry-immunofluorescence to suggest that sphingosine 1-phosphate receptor 1 is a major regulator of retinal neuron survival and restricts the ciliary neurotrophic factor-induced retinal ganglion cell growth response.

Mon Jul 30 00:00:00 EDT 2018

Sphingosine 1-phosphate receptor 1 is required for retinal ganglion cell survival after optic nerve trauma.

Journal of neurochemistry

Joly S,Pernet V

PA1-1040 was used in immunocytochemistry, immunohistochemistry - frozen section, and western blot to assess the requirement for retinal ganglion cell survival after optic nerve trauma and sphingosine 1-phosphate receptor 1

Mon Aug 01 00:00:00 EDT 2016

Stress-induced production of chemokines by hair follicles regulates the trafficking of dendritic cells in skin.

Nature immunology

Nagao K,Kobayashi T,Moro K,Ohyama M,Adachi T,Kitashima DY,Ueha S,Horiuchi K,Tanizaki H,Kabashima K,Kubo A,Cho YH,Clausen BE,Matsushima K,Suematsu M,Furtado GC,Lira SA,Farber JM,Udey MC,Amagai M

PA1-1040 was used in immunohistochemistry to study the regulatory role of stress-induced chemokines on dendritic cell trafficking in skin

Sun Jun 24 00:00:00 EDT 2012

A polysaccharide, MDG-1, induces S1P1 and bFGF expression and augments survival and angiogenesis in the ischemic heart.

Glycobiology

Wang S,Zhang Z,Lin X,Xu DS,Feng Y,Ding K

PA1-1040 was used in western blot to investigate the effect of the polysaccharide MDG-1 on specific gene expression and cell survival in ischemic hearts

Fri Jan 01 00:00:00 EST 2010

生物信息学

蛋白别名: CD antigen CD363; CD363; CD363 antigen; EDG1 (Edg1); Endothelial differentiation G-protein coupled receptor 1; endothelial differentiation sphingolipid G-protein-coupled receptor 1; endothelial differentiation, sphingolipid G-protein-coupled receptor, 1; Lysophospholipid receptor B1; S1P receptor 1; S1P receptor Edg-1; S1P1; Sphingosine 1-phosphate receptor 1; Sphingosine 1-phosphate receptor Edg-1; sphingosine 1-phosphate receptor EDG1

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基因别名: AI849002; CD363; CHEDG1; D1S3362; ECGF1; EDG-1; EDG1; Lpb1; S1p; S1P1; S1PR1

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UniProt ID: (Human) P21453, (Rat) P48303, (Mouse) O08530

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Entrez Gene ID: (Human) 1901, (Rat) 29733, (Mouse) 13609

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功能

G-protein coupled receptor binding G-protein coupled receptor activity sphingosine-1-phosphate receptor activity sphingolipid binding signal transducer activity G-protein coupled receptor

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