Invitrogen

S6 Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (8)
高级验证 (1)

S6 Antibody in Immunocytochemistry (ICC/IF)

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S6 Antibody (PA5-29760) in ICC/IF

Immunofluorescence analysis of S6 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with S6 Polyclonal Antibody (Product # PA5-29760) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (... View More

S6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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S6 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with S6 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-S6 Polyclonal Antibody (Product # PA5-29760). {KD}

产品信息

PA5-29760

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫组织化学（悬浮细胞） (IHC (Free))

Assay-dependent

免疫细胞化学 (ICC/IF)

1:100-1:1,000

产品规格

种属反应

Human, Mouse, Zebrafish

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein fragment corresponding to a region within amino acids 1 and 146 of Human RPS6

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2.34 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2547234

产品详细信息

Recommended positive controls: HeLa, HepG2, Mouse brain.

Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (96%), Drosophila (84%), Xenopus laevis (96%), Dog (100%), Chicken (98%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: 40S ribosomal protein S6; OTTHUMP00000021121; OTTHUMP00000021122; Phosphoprotein NP33; RP11-513M16.6; RS6; Small ribosomal subunit protein eS6

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基因别名: OK/SW-cl.2; RPS6; S6; S6R; wu:fa92e06; wu:fb64g06; zgc:92237

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UniProt ID: (Human) P62753, (Mouse) P62754

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Entrez Gene ID: (Human) 6194, (Mouse) 20104, (Zebrafish) 445028

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功能

structural constituent of ribosome protein binding protein kinase binding poly(A) RNA binding ribosomal protein translational protein

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