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Invitrogen
SATB1 Polyclonal Antibody
高级验证
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
图: 1 / 12
SATB1 Antibody (PA5-30163) in ICC/IF
SATB1 Polyclonal Antibody detects SATB1 protein at nucleus by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SATB1 stained by SATB1 Polyclonal Antibody (Product # PA5-30163) diluted at 1:500. Blue: Fluoroshield with DAPI .
产品信息
PA5-30163
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Not Applicable
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fragment corresponding to a region within amino acids 514 and 749 of Human SATB1
偶联物
形式
Liquid
浓度
0.36 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 1% BSA, 20% glycerol
内含物
0.025% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2547637
产品详细信息
Recommended positive controls: Jurkat, THP-1, mouse thymus.
Predicted reactivity: Mouse (96%), Rat (96%), Chicken (91%), Rhesus Monkey (99%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
SATB1 (DNA-binding protein SATB1) is a crucial silencing factor that contributes to the initiation of X inactiviation mediated by Xist RNA that occurs during embryogenesis and in lymphoma. SATB1 binds to DNA at special AT-rich sequences, the consensus SATB1-binding sequence, and at nuclear matrix- or scaffold-associated regions. It is thought to recognize the sugar-phosphate structure of double-stranded DNA. SATB1 is also a transcriptonal repressor controlling nuclear and viral gene expression in a phosphorylated and acetylated status-dependent manner by binding to matrix attachment regions of DNA and inducing a local chromatin-loop remodeling. SATB1 modulates the genes that are essential in the maturatio of the immune T-cell CD8SP from thymocytes. Alu-like motifs and SATB1-binding sites provide an unique chromatin context which seems preferentially targeted by the HIV-1 integration machinery. Moreover, HIV-1 Tat may overcome SATB1-mediated repression of IL1 and IL2RA in T-cells by binding to the same domain as HDAC1.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: DNA-binding protein SATB1; special AT-rich sequence binding protein 1 (binds to nuclear matrix/scaffold-associating DNA); Special AT-rich sequence-binding protein 1
基因别名: 2610306G12Rik; AW413156; SATB1
UniProt ID: (Human) Q01826, (Mouse) Q60611
Entrez Gene ID: (Human) 6304, (Mouse) 20230
RNA polymerase II regulatory region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
chromatin binding
double-stranded DNA binding
protein binding
DNA binding
transcription factor activity, sequence-specific DNA binding
helix-turn-helix transcription factor
DNA-binding transcription factor
Lambda repressor-like transcription factor
gene-specific transcriptional regulator
negative regulation of transcription from RNA polymerase II promoter
chromatin organization
chromatin remodeling
transcription, DNA-templated
cellular component disassembly involved in execution phase of apoptosis
epidermis development
viral process
histone methylation
CD4-positive, alpha-beta T cell differentiation
CD8-positive, alpha-beta T cell differentiation
activated T cell proliferation
reflex
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
chromatin modification
T cell activation
