Invitrogen

SAV1 Recombinant Rabbit Monoclonal Antibody (6H5L16)

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

查看其他12个SAV1抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (2)
高级验证 (1)

SAV1 Antibody in Immunocytochemistry (ICC/IF)

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SAV1 Antibody (703002) in ICC/IF

Knockdown of SAV1 was achieved by transfecting HCT 116 cells with specific siRNA (Silencer® select Product # s34083, s34081). Immunofluorescence analysis was performed on HCT 116 cells (untransfected, panel a-d), transfected with SAV1 specific siRNA (panel i-l) or non-specific scrambled siRNA (panels e-h). Cells were fixed, permeabilized, and labelled with Anti-SAV1 Recombinant Rabbit Monoclonal Antibody (Product # 703002, 1:100 dilution), followed by Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conj... View More

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SAV1 Antibody — Antibody specificity was demonstrated by siRNA-mediated knockdown of target protein. HCT 116 cells were transfected with SAV1 siRNA and decrease in signal intensity was observed in immunofluorescence application using Anti-SAV1 Recombinant Rabbit Monoclonal Antibody (Product # 703002). {KD}

产品信息

703002

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

6H5L16

抗原

Protein corresponding to human SAV1 [aa300-aa383]

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2762385

产品详细信息

This antibody is predicting to react with Monkey, Cat, Dog.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

Sav1 is a regulator of STK3/MST2 and STK4/MST1 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. SAV1 is required for STK3/MST2 and STK4/MST1 activation and promotes cell-cycle exit and terminal differentiation in developing epithelial tissues. It also plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosomes, and its ability to phosphorylate CROCC and CEP250. In conjunction with STK3/MST2, Sav1 activates the transcriptional activity of ESR1 through the modulation of its phosphorylation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。