Invitrogen

SAV1 Monoclonal Antibody (OTI2B7)

SAV1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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SAV1 Antibody (MA5-26689) in IHC (P)

Immunohistochemistry was performed on paraffin-embedded human kidney tissue. To expose target proteins, 1 mM EDTA in 10mM Tris, pH8.0 was used. Following antigen retrieval, tissues were probed with a SAV1 monoclonal antibody (Product # MA5-26689) at a dilution of 1:150.

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产品信息

MA5-26689

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

免疫组化（石蜡） (IHC (P))

1:150

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

OTI2B7

抗原

Human recombinant protein fragment corresponding to amino acids 167-383 of SAV1 produced in E.coli

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 1% BSA, 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2725297

靶标信息

Sav1 is a regulator of STK3/MST2 and STK4/MST1 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. SAV1 is required for STK3/MST2 and STK4/MST1 activation and promotes cell-cycle exit and terminal differentiation in developing epithelial tissues. It also plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosomes, and its ability to phosphorylate CROCC and CEP250. In conjunction with STK3/MST2, Sav1 activates the transcriptional activity of ESR1 through the modulation of its phosphorylation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。