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SCF Antibody (500-P202BT-25UG) in WB
Western Blot: To detect Rat SCF by Western Blot analysis SCF Polyclonal Antibody, Biotin (Product # 500-P202BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat SCF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
产品信息
500-P202BT-25UG
产品规格
种属反应
Rat
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived, 18.4 kDa Recombinant Rat SCF
偶联物
形式
Lyophilized
浓度
0.1-1.0 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
AB_2929801
产品详细信息
AA Sequence of recombinant protein: MQEICRNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV THLSVSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVAC MEENAPKNVK ESLKKPETRN FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Rat SCF. Anti-Rat SCF-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect rat SCF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Rat SCF (500-P202) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant rat SCF.
Western Blot: To detect Rat SCF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat SCF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P202BT-1MG will be provided as 2 x 500 µg
靶标信息
Stem cell factor (SCF) is the ligand of the c-Kit oncogene and is expressed by various structural and inflammatory cells in the airways. Binding of SCF by the c-Kit receptor leads to homodimerization of the receptor and the activation of signalling pathways such as PI-3, PLC-gamma, Jak/STAT, and MAP kinase pathways. SCF expression leads to the induction of mast cell survival and the expression and release of histamine, pro-inflammatory cytokines and chemokines. The inhibition of the SCF/c-Kit pathway leads to a decrease in histamine levels, mast cell and eosinophil infiltration, IL-4 production and airway hyperresponsiveness, suggesting this pathway may be a useful therapeutic target in inflammatory diseases such as asthma. At least two isoforms of SCF are known to exist.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: C-kit ligand; DKFZp686F2250; H-SCF; Hematopoietic growth factor KL; Kit ligand; kit-ligand; M-SCF; Mast cell growth factor; MGF; SCF; steel factor/kit ligand; Stem cell factor; stem cell factor KL-1
基因别名: Kitl; Kitlg; Mgf; SCF
UniProt ID: (Rat) P21581
Entrez Gene ID: (Rat) 60427
ovarian follicle development
neural crest cell migration
positive regulation of myeloid leukocyte differentiation
cell adhesion
positive regulation of cell proliferation
male gonad development
negative regulation of mast cell apoptotic process
embryonic hemopoiesis
ectopic germ cell programmed cell death
negative regulation of apoptotic process
positive regulation of MAP kinase activity
positive regulation of melanocyte differentiation
positive regulation of DNA replication
positive regulation of Ras protein signal transduction
positive regulation of peptidyl-tyrosine phosphorylation
positive regulation of mast cell proliferation
extrinsic apoptotic signaling pathway in absence of ligand
