Invitrogen

SEMA4A Monoclonal Antibody (KL-1 (aka SK31)), eBioscience™

产品信息

14-1002-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

5 µg/mL

体外检测 (IV)

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产品规格

种属反应

Mouse

已发表种属

Rat

宿主/亚型

Rat / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

KL-1 (aka SK31)

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10547286

产品详细信息

Description: The KL-1 (aka SK31) antibody reacts with mouse Sema4A, a class IV semaphorin originally identified as SemB. Sema4A is structurally similar to Sema4D/CD100. Sema4A is reported to be expressed by dendritic cells, B cells and activated T cells. Although published data suggested that Sema4A binds to Tim-2, a T cell surface receptor, it remains to be definitively shown that these two molecules have a receptor/ligand relation. It is suggested that Sema4a can provide costimulatory signals for T cells.

This KL-1 antibody works in immunoblotting but requires non-reducing conditions.

Applications Reported: This KL-1 (aka SK31) antibody has been reported for use in immunoblotting (WB).

Applications Tested: The KL-1 (aka SK31) antibody has been tested by immunoblot analysis of mouse Sema4A transfected cells under nonreducing conditions. This can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

SEMA4A is a member of the semaphorin family of soluble and transmembrane proteins. Semaphorins are involved in guidance of axonal migration during neuronal development and in immune responses.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

MHC/class-II-positive cells inhibit corticosterone of adrenal gland cells in experimental arthritis: a role for IL-1β, IL-18, and the inflammasome.

Scientific reports

Stangl H,Krammetsvogl A,Lesiak M,Wolff C,Straub RH

14-1002-82 was used in In vitro Assay to show that activated macrophages and/or dendritic cells inhibit glucocorticoid secretion in experimental arthritis and that IL-1β is a decisive factor.