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SF1 Antibody (MA5-49178) in ICC/IF
Immunocytochemistry analysis of SF1 in A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in SF1 Monoclonal antibody (Product # MA5-49178) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the... View More
产品信息
MA5-49178
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
7D9E3
抗原
E. coli-derived human splicing factor 1 recombinant protein (Position: R160-Q266).
偶联物
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_3074836
产品详细信息
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human SKOV-3 whole cell, human K562 whole cell, human THP-1 whole cell, human A549 whole cell, rat PC-12 whole cell, mouse ovary tissue, mouse NIH/3T3 whole cell. IHC: human bladder cancer tissue, human breast cancer tissue, human liver cancer tissue, human placenta tissue, human squamous metaplasia of the renal pelvis tissue, mouse colon tissue, rat colon tissue. ICC/IF: A431 cell. Flow: Neuro-2a cell, Neuro-2a cell, C6 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
Splicing factor 1 is a nuclear pre-mRNA splicing factor. The protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: BBP; M ZFM1; Mammalian branch point-binding protein; SF; Splicing factor 1; Transcription factor ZFM1; Zinc finger gene in MEN1 locus; Zinc finger protein 162
基因别名: BBP; D11S636; MBBP; MZFM; SF1; WBP4; ZCCHC25; ZFM1; Zfp162; ZNF162
UniProt ID: (Human) Q15637
Entrez Gene ID: (Human) 7536, (Mouse) 22668, (Rat) 117855
spliceosomal complex assembly
mRNA 3'-splice site recognition
mRNA splicing, via spliceosome
transcription, DNA-templated
regulation of transcription, DNA-templated
male sex determination
Leydig cell differentiation
negative regulation of smooth muscle cell proliferation
regulation of steroid biosynthetic process
negative regulation of nucleic acid-templated transcription
mRNA processing
RNA splicing
