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SLC38A1 Antibody (MA5-45529) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with SNAT1 monoclonal antibody (Product # MA5-45529) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for 60 min RT, 5 min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin sta... View More
产品信息
MA5-45529
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
N104/32
抗原
Fusion protein amino acids 1- 63 of rat SNAT1
偶联物
激发/发射光谱
651/660 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
内含物
no preservative
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2931983
产品详细信息
1 µg/mL of MA5-45529 was sufficient for detection of SNAT1 in 20 µg of lysates from neocortical neurons cultured under amino acid starvation conditions and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 50kDa.
This antibody was formerly sold as clone S104-32.
靶标信息
Amino acid transporters play essential roles in the uptake of nutrients, production of energy, chemical metabolism, detoxification, and neurotransmitter cycling. SLC38A1 is an important transporter of glutamine, an intermediate in the detoxification of ammonia and the production of urea. Glutamine serves as a precursor for the synaptic transmitter, glutamate.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Amino acid transporter A1; amino acid transporter system A1; Glutamine transporter; MNat2; N-system amino acid transporter 2; rATA1; S38A1; SNAT 1; Sodium-coupled neutral amino acid symporter 1; sodium-coupled neutral amino acid transporter 1; Solute carrier family 38 member 1; solute carrier family 38, member 1; System A amino acid transporter 1; System A transporter 2; System N amino acid transporter 1
基因别名: AA408026; AA409865; AL022800; ATA1; AU015942; GlnT; NAT2; Sa2; SAT1; SLC38A1; SNAT1
UniProt ID: (Human) Q9H2H9, (Rat) Q9JM15, (Mouse) Q8K2P7
Entrez Gene ID: (Human) 81539, (Rat) 170567, (Mouse) 105727
sodium:amino acid symporter activity
L-histidine transmembrane transporter activity
protein binding
amino acid transmembrane transporter activity
neutral amino acid transmembrane transporter activity
L-amino acid transmembrane transporter activity
L-asparagine transmembrane transporter activity
L-glutamine transmembrane transporter activity
symporter activity
amino acid transporter
neurotransmitter uptake
sodium ion transport
amino acid transport
asparagine transport
glutamine transport
neutral amino acid transport
L-amino acid transport
histidine transport
L-histidine transmembrane transport
amino acid transmembrane transport
cation transmembrane transport
L-alpha-amino acid transmembrane transport
transport
ion transport
