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SLIT3 Antibody (600-401-884) in WB
Western blot using Rocklands Affinity Purified anti-SLIT-3 antibody shows detection of a predominant band at ~145 kDa corresponding to SLIT-3 (arrowhead) in a bovine thyroid whole cell lysate using the 800 nm channel (green). ~ 35 µg of lysate was separated on a 4-8% Tricine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:800. Incubation was for 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Ra... View More
产品信息
600-401-884
产品规格
种属反应
Bovine,
Dog,
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 1155-1180 of Human SLIT-3 protein.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
内含物
0.01% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against human SLIT-3 protein. A BLAST analysis was used to suggest reactivity with this protein from human, rat, dog, bovine and mouse based on 100% homology for the immunogen sequence. Expect cross reactivity with SLIT-3 from chimpanzee and chicken sources, as only a single amino acid residue change is found within the immunogen sequence (93% positive by BLAST). Cross reactivity with SLIT-3 homologues from other sources has not been determined.
靶标信息
SLIT3 may act as molecular guidance cue in cellular migration, and function may be mediated by interaction with roundabout homolog receptors.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Multiple EGF-like domains protein 5; multiple epidermal growth factor-like domains 5; Multiple epidermal growth factor-like domains protein 5; SLIL2FLJ10764; slit homolog 3; Slit homolog 3 protein; Slit-3
基因别名: b2b2362.1Clo; KIAA0814; MEGF5; SLIL2; Slit-3; SLIT1; slit2; SLIT3; UNQ691/PRO1336
UniProt ID: (Human) O75094, (Rat) O88280, (Mouse) Q9WVB4
Entrez Gene ID: (Dog) 489132, (Bovine) 615883, (Human) 6586, (Rat) 83467, (Mouse) 20564
axon guidance
negative regulation of cell proliferation
organ morphogenesis
negative regulation of gene expression
chemorepulsion involved in embryonic olfactory bulb interneuron precursor migration
negative regulation of cell growth
cellular response to hormone stimulus
Roundabout signaling pathway
axon extension involved in axon guidance
regulation of axonogenesis
negative chemotaxis
response to cortisol
apoptotic process involved in luteolysis
negative regulation of chemokine-mediated signaling pathway
brain development
spinal cord development
cell differentiation
multicellular organism development
nervous system development
