Invitrogen

SMAD3 Monoclonal Antibody (5G11)

SMAD3 Antibody in Immunocytochemistry (ICC/IF)

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SMAD3 Antibody (MA5-15663) in ICC/IF

Immunofluorescence analysis of NIH/3T3 cells using SMAD3 monoclonal antibody (Product # MA5-15663) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

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产品信息

MA5-15663

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

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免疫组化 (IHC)

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免疫细胞化学 (ICC/IF)

1:200-1:1,000

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酶联免疫吸附实验 (ELISA)

1:10,000

in situ PLA (PLA)

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其他PubMed (Misc)

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产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

5G11

抗原

Purified recombinant fragment of human SMAD3 expressed in E. Coli.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. Not Determined

保存液

ascites

内含物

0.03% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_10983274

产品详细信息

MA5-15663 targets SMAD3 in indirect ELISA, ICC, IF and WB applications and shows reactivity with Human and mouse samples.

The MA5-15663 immunogen is purified recombinant fragment of human SMAD3 expressed in E. Coli.
.

MA5-15663 detects SMAD3 which has a predicted molecular weight of approximately 48kDa.

靶标信息

SMAD3 belongs to the SMAD family of proteins. They are similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Core transcription regulatory circuitry orchestrates corneal epithelial homeostasis.

Nature communications

Li M,Huang H,Li L,He C,Zhu L,Guo H,Wang L,Liu J,Wu S,Liu J,Xu T,Mao Z,Cao N,Zhang K,Lan F,Ding J,Yuan J,Liu Y,Ouyang H

MA5-15663 was used in Western Blotting to highlight CRC TF cooperativity for establishment of stem cell identity and lineage commitment, and provides comprehensive regulatory principles for human stratified epithelial homeostasis and pathogenesis.

Mon Jan 18 00:00:00 EST 2021

Copy number variation and expression of exportin-4 associates with severity of fibrosis in metabolic associated fatty liver disease.

EBioMedicine

Metwally M,Bayoumi A,Khan A,Adams LA,Aller R,García-Monzón C,Arias-Loste MT,Bugianesi E,Miele L,Anna A,Latchoumanin O,Han S,Alenizi S,Sharkawy RE,Elattar A,Gallego-Durán R,Fischer J,Berg T,Liddle C,Romero-Gomez M,George J,Eslam M

MA5-15663 was used in Proximity Ligation Assay to investigate if duplication in the exportin 4 (XPO4) copy number variations is associated with the severity of liver fibrosis.

Sun Aug 01 00:00:00 EDT 2021

SOX4 can redirect TGF-β-mediated SMAD3-transcriptional output in a context-dependent manner to promote tumorigenesis.

Nucleic acids research

Vervoort SJ,Lourenço AR,Tufegdzic Vidakovic A,Mocholi E,Sandoval JL,Rueda OM,Frederiks C,Pals C,Peeters JGC,Caldas C,Bruna A,Coffer PJ

Published figure using SMAD3 monoclonal antibody (Product # MA5-15663) in Immunofluorescence

Fri Oct 12 00:00:00 EDT 2018

SOX4 can redirect TGF-β-mediated SMAD3-transcriptional output in a context-dependent manner to promote tumorigenesis.

Nucleic acids research

Vervoort SJ,Lourenço AR,Tufegdzic Vidakovic A,Mocholi E,Sandoval JL,Rueda OM,Frederiks C,Pals C,Peeters JGC,Caldas C,Bruna A,Coffer PJ

Published figure using SMAD3 monoclonal antibody (Product # MA5-15663) in Immunofluorescence

Fri Oct 12 00:00:00 EDT 2018

RIPK1-RIPK3-MLKL-dependent necrosis promotes the aging of mouse male reproductive system.

eLife

Li D,Meng L,Xu T,Su Y,Liu X,Zhang Z,Wang X

MA5-15663 was used in Immunohistochemistry to report that male reproductive organs of both Ripk3- and Mlkl-knockout mice retain ''youthful'' morphology and function into advanced age, while those of age-matched wild-type mice deteriorate.

Tue Aug 15 00:00:00 EDT 2017

Soluble MMP-14 produced by bone marrow-derived stromal cells sheds epithelial endoglin modulating the migratory properties of human breast cancer cells.

Carcinogenesis

Tobar N,Avalos MC,Méndez N,Smith PC,Bernabeu C,Quintanilla M,Martínez J

MA5-15663 was used in western blot to study the modulation of breast cancer cell migratory potential by endoglin shed from epithelial cells by soluble MMP14 from bone marrow-derived stromal cells

Fri Aug 01 00:00:00 EDT 2014

c-Jun N terminal kinase modulates NOX-4 derived ROS production and myofibroblasts differentiation in human breast stromal cells.

BMC cancer

Tobar N,Toyos M,Urra C,Méndez N,Arancibia R,Smith PC,Martínez J

MA5-15663 was used in western blot to examine the role of c-Jun N terminal kinase in myofibroblasts differentiation in human breast stromal cells

Sat Aug 30 00:00:00 EDT 2014

生物信息学

蛋白别名: DKFZp586N0721; DKFZp686J10186; hMAD-3; hSMAD3; JV15-2; MAD homolog 3; mad homolog JV15-2; mad protein homolog; MAD, mothers against decapentaplegic homolog 3; MAD-3; mad3; MGC60396; mMad3; Mothers against decapentaplegic homolog 3; mothers against DPP homolog 3; SMA- and MAD-related protein 3; SMAD; SMAD 3; SMAD family member 3; SMAD, mothers against DPP homolog 3

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基因别名: AU022421; HSPC193; HsT17436; JV15-2; LDS1C; LDS3; MADH3; SMAD3

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UniProt ID: (Human) P84022, (Mouse) Q8BUN5

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Entrez Gene ID: (Human) 4088, (Mouse) 17127

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