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SMAD5 Antibody (PA5-78050) in ICC/IF
Immunofluorescence analysis of SMAD5 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with SMAD5 Polyclonal Antibody (Product # PA5-78050) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A327... View More
产品信息
PA5-78050
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein encompassing a sequence within the center region of human SMAD5. The exact sequence is proprietary.
偶联物
形式
Liquid
浓度
1.23 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 20% glycerol, 1% BSA
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2736631
产品详细信息
Positive Control: Target recombinant protein
Predicted Reactivity: Mouse (97%), Rat (99%), Chicken (93%), Rhesus Monkey (100%), Bovine (100%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily. SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, 2, 5, 8 and 9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and 7). SMAD1, 5, 8 and 9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. SMAD8 and SMAD9 are typically used as alternate names for one another in the literature.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DKFZp781C1895; DKFZp781O1323; hSmad5; JV5-1; MAD homolog 5; MAD, mothers against decapentaplegic homolog 5; Mothers against decapentaplegic homolog 5; mothers against decapentaplegic, drosophila, homolog of, 5; OTTHUMP00000223331; SMA- and MAD-related protein 5; SMAD; SMAD 5; SMAD family member 5; SMAD, mothers against DPP homolog 5
基因别名: DWFC; JV5-1; MADH5; SMAD5
UniProt ID: (Human) Q99717
Entrez Gene ID: (Human) 4090
RNA polymerase II core promoter sequence-specific DNA binding
transcription factor activity, sequence-specific DNA binding
receptor signaling protein activity
protein binding
transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity
ubiquitin protein ligase binding
metal ion binding
DNA-binding transcription factor
gene-specific transcriptional regulator
ureteric bud development
Mullerian duct regression
osteoblast fate commitment
transcription, DNA-templated
protein phosphorylation
signal transduction
transforming growth factor beta receptor signaling pathway
germ cell development
embryonic pattern specification
erythrocyte differentiation
BMP signaling pathway
positive regulation of osteoblast differentiation
positive regulation of transcription, DNA-templated
cartilage development
cardiac muscle contraction
bone development
SMAD protein signal transduction
cellular response to organic cyclic compound
positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus
