Invitrogen

SMO Polyclonal Antibody

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抗体检测数据 (3)

SMO Antibody in Immunocytochemistry (ICC/IF)

SMO Antibody in Immunocytochemistry (ICC/IF)

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SMO Antibody (PA5-113312) in ICC/IF

Immunocytochemichal analysis of SMO in A549 cells using a Polyclonal antibody (Product # PA5-113312) at a dilution of 1:100. An Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L) secondary antibdoy was used.

SMO Antibody in Immunocytochemistry (ICC/IF)

SMO Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SMO Antibody in Western Blot (WB)

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SMO Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemichal analysis of SMO in A549 cells using a Polyclonal antibody (Product # PA5-113312) at a dilution of 1:100. An Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L) secondary antibdoy was used.

SMO Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunocytochemical analysis of paraffin-embeded SMO in human liver tissue using a SMO Polyclonal antibody (Product # PA5-113312) at a dilution of 1:100.

SMO Antibody in Western Blot (WB) — Western Blot analysis of SMO in multiple sample types using a SMO polyclonal antibody (Product # PA5-113312) at a concentration of 2 µg/ml. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:50,000. Sample types that were tested include: Mouse liver tissue, Mouse kidney tissue .

产品信息

PA5-113312

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:5,000

-

免疫组化（石蜡） (IHC (P))

1:20-1:200

-

免疫细胞化学 (ICC/IF)

1:50-1:200

-

酶联免疫吸附实验 (ELISA)

Assay-dependent

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant Human Smoothened homolog protein (28-233AA)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.03% ProClin 300

保存条件

-20°C or -80°C if preferred

运输条件

Wet ice

RRID

AB_2868046

靶标信息

Smoothened drosophila homolog (SMOH) is a serpentine protein belonging to the G-protein coupled receptor Fz/Smo family with 787 aa, seven transmembrane G protein-coupled receptors with 4 glycosylation sites and a putative extracellular amino terminus. Smoothened activated by b-Arrestin 2 and GRK2 acts as a receptor for Shh, the Sonic hedgehog gene product, and is a positive regulator of hedgehog signaling pathway along with the smoothened inhibitor patched protein (PTCH) and regulates vertebrate developmental processes, cellular proliferation and differentiation by activating transcription factor Gli. Smoothened mutants are known to cause sporadic basal cell carcinoma of the skin. It is known to express widely in heart and brain.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: bent body; frizzled family member 11; HGNC:11119; Protein Gx; Protein SMOH; Protein smoothened; seven transmembrane helix receptor; smoothened homolog; smoothened, frizzled family receptor; smoothened, seven transmembrane spanning receptor

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基因别名: bnb; E130215L21Rik; FZD11; Gx; SMO; SMOH; smoothened

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UniProt ID: (Human) Q99835, (Mouse) P56726

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Entrez Gene ID: (Human) 6608, (Mouse) 319757

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G-protein coupled receptor activity patched binding protein binding drug binding Wnt-protein binding Wnt-activated receptor activity signal transducer activity transmembrane signaling receptor activity transmembrane signal receptor

参与通路

negative regulation of transcription from RNA polymerase II promoter vasculogenesis osteoblast differentiation in utero embryonic development cell fate specification neural crest cell migration heart looping positive regulation of neuroblast proliferation positive regulation of mesenchymal cell proliferation determination of left/right asymmetry in lateral mesoderm type B pancreatic cell development G-protein coupled receptor signaling pathway smoothened signaling pathway positive regulation of hh target transcription factor activity ventral midline determination midgut development anterior/posterior pattern specification positive regulation of gene expression negative regulation of gene expression dentate gyrus development cerebellar cortex morphogenesis thalamus development dorsal/ventral neural tube patterning smoothened signaling pathway involved in ventral spinal cord patterning smoothened signaling pathway involved in regulation of cerebellar granule cell precursor cell proliferation central nervous system neuron differentiation cerebral cortex development negative regulation of epithelial cell differentiation hair follicle morphogenesis protein localization to nucleus multicellular organism growth non-canonical Wnt signaling pathway positive regulation of multicellular organism growth positive regulation of protein import into nucleus odontogenesis of dentin-containing tooth negative regulation of apoptotic process negative regulation of DNA binding positive regulation of smoothened signaling pathway positive regulation of transcription from RNA polymerase II promoter positive regulation of organ growth astrocyte activation skeletal muscle fiber development forebrain morphogenesis homeostasis of number of cells within a tissue positive regulation of epithelial cell proliferation protein stabilization myoblast migration negative regulation of hair follicle development canonical Wnt signaling pathway detection of cell density by contact stimulus involved in contact inhibition atrial septum morphogenesis mammary gland epithelial cell differentiation epithelial-mesenchymal cell signaling somite development pancreas morphogenesis left/right axis specification cellular response to cholesterol renal system development mesenchymal to epithelial transition involved in metanephric renal vesicle formation positive regulation of branching involved in ureteric bud morphogenesis regulation of stem cell population maintenance regulation of heart morphogenesis ossification heart morphogenesis signal transduction cell surface receptor signaling pathway multicellular organism development determination of left/right symmetry pattern specification process central nervous system development positive regulation of cell proliferation dorsal/ventral pattern formation regulation of gene expression cell development digestive tract development embryonic organ development developmental growth axon extension involved in axon guidance