Invitrogen

SMYD2 Polyclonal Antibody

查看其他14个SMYD2抗体

抗体检测数据 (2)

SMYD2 Antibody in Immunocytochemistry (ICC/IF)

SMYD2 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 2

SMYD2 Antibody (PA5-121006) in ICC/IF

Immunofluorescence analysis of SMYD2 in U2OS cells. Samples were incubated with SMYD2 Polyclonal antibody (Product # PA5-121006). Blue: DAPI for nuclear staining.

SMYD2 Antibody in Immunocytochemistry (ICC/IF)

SMYD2 Antibody in Western Blot (WB)

Product Image Gallery

SMYD2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of SMYD2 in U2OS cells. Samples were incubated with SMYD2 Polyclonal antibody (Product # PA5-121006). Blue: DAPI for nuclear staining.

SMYD2 Antibody in Western Blot (WB) — Western blot analysis of SMYD2 in extracts of various cell lines. Samples were incubated with SMYD2 Polyclonal antibody (Product # PA5-121006) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

产品信息

PA5-121006

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

免疫细胞化学 (ICC/IF)

1:10-1:100

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human SMYD2 (NP_064582.2).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.77 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2914578

产品详细信息

Positive test controls include: HeLa, 293T, Mouse heart, Mouse brain. The target is usually found in the following locations: Cytoplasm, Nucleus, cytosol.

Immunogen sequence: MRAEGLGGLE RFCSPGKGRG LRALQPFQVG DLLFSCPAYA YVLTVNERGN HCEYCFTRKE GLSKCGRCKQ AFYCNVECQK EDWPMHKLEC SPMVVFGENW NPSETVRLTA RILAKQKIHP ERTPSEKLLA VKEFESHLDK LDNEKKDLIQ SDIAALHHFY SKHLGFPDND SLVVLFAQVN CNGFTIEDEE LSHLGSAIFP DVALMNHSCC PNVIVTYKGT LAEVRAVQEI KPGEEVFTSY IDLLYPTEDR NDRLRDSYFF TCECQECTTK

靶标信息

SET domain-containing proteins, such as SMYD2, catalyze lysine methylation..

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: Histone methyltransferase SMYD2; HSKM-B; Lysine N-methyltransferase 3C; N-lysine methyltransferase SMYD2; SET and MYND domain-containing protein 2; zinc finger, MYND domain containing 14

查看更多收起

基因别名: 1110020E07Rik; 4930402C15; HSKM-B; KMT3C; SMYD2; ZMYND14

查看更多收起

UniProt ID: (Human) Q9NRG4, (Mouse) Q8R5A0

查看更多收起

Entrez Gene ID: (Human) 56950, (Mouse) 226830

查看更多收起

RNA polymerase II core binding p53 binding protein binding lysine N-methyltransferase activity protein-lysine N-methyltransferase activity histone-lysine N-methyltransferase activity metal ion binding histone methyltransferase activity (H3-K36 specific) rRNA (adenine-N6,N6-)-dimethyltransferase activity protein C-terminal carboxyl O-methyltransferase activity tRNA (guanine-N2-)-methyltransferase activity methyltransferase activity C-methyltransferase activity N-methyltransferase activity O-methyltransferase activity S-methyltransferase activity RNA methyltransferase activity mRNA methyltransferase activity tRNA methyltransferase activity protein methyltransferase activity 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity rRNA methyltransferase activity rRNA (uridine-2'-O-)-methyltransferase activity rRNA (adenine-N6-)-methyltransferase activity DNA-methyltransferase activity tRNA (guanine-N1-)-methyltransferase activity rRNA (cytosine-C5-)-methyltransferase activity selenocysteine methyltransferase activity arginine N-methyltransferase activity protein-arginine N-methyltransferase activity tRNA (uracil) methyltransferase activity tRNA (guanine) methyltransferase activity tRNA (adenine) methyltransferase activity tRNA (cytosine) methyltransferase activity tRNA (cytosine-5-)-methyltransferase activity tRNA (adenine-N1-)-methyltransferase activity rRNA (adenine) methyltransferase activity rRNA (cytosine) methyltransferase activity rRNA (guanine) methyltransferase activity rRNA (uridine) methyltransferase activity transferase activity protein C-terminal leucine carboxyl O-methyltransferase activity 1-phenanthrol methyltransferase activity protein-arginine N5-methyltransferase activity methylarsonite methyltransferase activity dimethylarsinite methyltransferase activity 4,5-dihydroxybenzo(a)pyrene methyltransferase activity 1-hydroxypyrene methyltransferase activity 1-hydroxy-6-methoxypyrene methyltransferase activity demethylmenaquinone methyltransferase activity cobalt-precorrin-6B C5-methyltransferase activity cobalt-precorrin-7 C15-methyltransferase activity cobalt-precorrin-5B C1-methyltransferase activity cobalt-precorrin-3 C17-methyltransferase activity dimethylamine methyltransferase activity hydroxyneurosporene-O-methyltransferase activity tRNA (adenine-57, 58-N(1)-) methyltransferase activity methylamine-specific methylcobalamin:coenzyme M methyltransferase activity trimethylamine methyltransferase activity methanol-specific methylcobalamin:coenzyme M methyltransferase activity monomethylamine methyltransferase activity P-methyltransferase activity Se-methyltransferase activity 2-phytyl-1,4-naphthoquinone methyltransferase activity tRNA (uracil-2'-O-)-methyltransferase activity tRNA (cytosine-2'-O-)-methyltransferase activity phosphomethylethanolamine N-methyltransferase activity tRNA (cytosine-3-)-methyltransferase activity rRNA (cytosine-2'-O-)-methyltransferase activity rRNA (cytosine-N4-)-methyltransferase activity trihydroxyferuloyl spermidine O-methyltransferase activity histone modifying enzyme

参与通路

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated heart development negative regulation of cell proliferation histone H3-K36 methylation peptidyl-lysine monomethylation peptidyl-lysine dimethylation regulation of DNA damage response, signal transduction by p53 class mediator regulation of signal transduction by p53 class mediator regulation of transcription, DNA-templated chromatin modification histone methylation methylation