Invitrogen

SNX27 Monoclonal Antibody (1C6)

SNX27 Antibody in Immunocytochemistry (ICC/IF)

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SNX27 Antibody (MA5-27854) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of SNX27 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: SNX27 Monoclonal Antibody (1C6) (Product # MA5-27854) diluted at 1:200. Blue: Hoechst 33342 staining.

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产品信息

MA5-27854

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

查看2篇已发表文章 2篇已发表文章

免疫细胞化学 (ICC/IF)

1:100-1:1,000

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

1C6

抗原

Human SNX27

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.37 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2735367

产品详细信息

Positive Control: 293T, A431, HeLa, HepG2

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

SNX27 encodes a member of the sorting nexin family, a diverse group of cytoplasmic and membrane-associated proteins involved in endocytosis of plasma membrane receptors and protein trafficking through these compartments. All members of this protein family contain a phosphoinositide binding domain (PX domain). A highly similar protein in mouse is responsible for the specific recruitment of an isoform of serotonin 5-hydroxytryptamine 4 receptor into early endosomes, suggesting the analogous role for the human protein.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Native holdup (nHU) to measure binding affinities from cell extracts.

Science advances

Zambo B,Morlet B,Negroni L,Trave G,Gogl G

Published figure using SNX27 monoclonal antibody (Product # MA5-27854) in Western Blot

Wed Dec 21 00:00:00 EST 2022

Quantitative fragmentomics allow affinity mapping of interactomes.

Nature communications

Gogl G,Zambo B,Kostmann C,Cousido-Siah A,Morlet B,Durbesson F,Negroni L,Eberling P,Jané P,Nominé Y,Zeke A,Østergaard S,Monsellier É,Vincentelli R,Travé G

MA5-27854 was used in Western Blotting to show that interactome hijacking by the viral PBM of human papillomavirus E6 oncoprotein substantially impacts the host cell proteome beyond immediate E6 binders, illustrating the complex system-wide relationship between interactome and function.