Invitrogen

SOX11 Monoclonal Antibody (SOX11-C1), Biotin, eBioscience™

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SOX11 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SOX11 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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SOX11 Antibody (13-9773-82) in IHC (P)

Immunohistochemistry of formalin-fixed paraffin embedded human mantle cell lymphoma using 10 µg/mL of Mouse IgG1 K Isotype Control Biotin (left) or 10 µg/mL of Anti-Human Sox11 Biotin (right) followed by Streptavidin HRP and DAB visualization. Nuclei are counterstained with hematoxylin.

SOX11 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SOX11 Antibody in Western Blot (WB)

SOX11 Antibody in Western Blot (WB)

SOX11 Antibody in Western Blot (WB)

SOX11 Antibody in Western Blot (WB)

Product Image Gallery

SOX11 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of formalin-fixed paraffin embedded human mantle cell lymphoma using 10 µg/mL of Mouse IgG1 K Isotype Control Biotin (left) or 10 µg/mL of Anti-Human Sox11 Biotin (right) followed by Streptavidin HRP and DAB visualization. Nuclei are counterstained with hematoxylin.

SOX11 Antibody in Western Blot (WB) — Figure 3. SOX11 expression promotes expression of developmental pathways frequently activated in cancer. ( A ) Volcano plot representing the RNAs with a log2 fold-change > +/- 0.585 in the RNA-sequencing results of iSOX11 cells grown in 2D compared with the controls [(iSOX11-niSOX11)- (iEV-niEV)] to account for effects of DOX treatment on DCIS.com cells. ( B ) Gene ontology results from A. ( C ) List of genes overexpressed log2 fold-change >+/-1.585 times in all three RNA-sequencing (cells grown in: 2D, 3D for 2 days, 3D for 5 days) results comparing iSOX11 versus iEV. ( D ) qRT-PCR results for several potential SOX11 targets in EV and SOX11 cells with and without DOX induction in cells grown in 2D. Experiment was repeated three times. ( E ) Western blot of MEX3A and TUBB3 in cytoplasmic and nuclear fractions of EV or SOX11 cells in presence or absence of 1 muM DOX. GAPDH and LAMIN B1 were used as loading control of cytoplasmic and nuclear fractions respectively. In brackets, densitometry results normalised against niEV and niSOX11. ( F ) IF staining of DCIS iEV and DCIS iSOX11 cells with TUBB3 (green) and MEX3A (red). Scale: 200 mum. ( G ) Western blot of MEX3A and TUBB3 in SOX11+ breast cancer cell lines and SOX11- DCIS.com and MCF10A from the MCF10A mammary cell progression series. ( H ) Pie charts representing the percentage of breast cancer samples with a log2 fold-change greater than two in the levels of MEX3A or TUBB3 RNA when SOX11 increased between 0.5- and 2-fold, 2

SOX11 Antibody in Western Blot (WB) — Figure 1. Inducible expression of SOX11 leads to changes in cell state profiles of DCIS.com cells. ( A ) Western blot of SOX11 in cytoplasmic and nuclear fractions of DCIS.com cells containing the pInducer21 empty vector in presence (iEV) or absence (niEV) of 1 muM Doxycycline (DOX) or the pInducer21SOX11 with (iSOX11) or without DOX (niSOX11). GAPDH and LAMIN B1 were used as loading control of cytoplasmic and nuclear fractions, respectively. Densitometry results normalised against niSOX11 are shown in brackets. ( B ) SOX11 expression detected in iSOX11 cells stained by IF after 48 hr of DOX induction. Scale Bar: 200 mum. ( C ) ER- DCIS case sample showing SOX11 staining in DCIS and adjacent normal breast tissue. Scale Bar: 200 mum. ( D ) Results from flow cytometry analysis of Aldefluor assays of niEV and niSOX11 cells (day 0) and iEV and iSOX11 after 2 days treatment with 1 muM DOX. Results show the % of ALDH+ cells normalised against niEV. Error bars represent SD. *p = 0.0223. n = 5. ( E ) Results from flow cytometry analysis of CD24 and CD44 of niEV and niSOX11 cells (day 0) and iEV and iSOX11 after treatment with 1 muM DOX for 2 days. Results show the average % of cells CD44+/CD24+ in each condition. Error bars represent SD. ***p = 0.0005 (iSOX11 vs niSOX11) and p = 0.0009 (iSOX11 vs iEV) n = 3. ( F ) Western blot of CD24 in cytoplasmic and nuclear fractions of niEV, niSOX11, iEV and iSOX11 cells. GAPDH and LAMIN B1 were used as loading control of cytoplasmic and nuclear

SOX11 Antibody in Western Blot (WB) — Figure 6. SOX11 regulates growth of ER- breast cancer cells. ( A ) qRT-PCR results for several potential SOX11 targets in CAL-148 cells transduced with shRNA to SOX11 or shRNA NS cells with and without DOX induction in cells grown in 2D. ( B ) Western blot of SOX11, MEX3A and TUBB3 in total cell lysates of CAL-148 cells transduced with shRNA SOX11 in presence or absence of 1 muM DOX after 48 hr. GAPDH was used as loading control. Densitometry results normalised against no DOX are shown in brackets. ( C ) Cell growth assay results for CAL-148 shRNA SOX11 cells induced with 1 muM DOX at 3 and 7 days. Experiments were performed three times. Error bars represent SEM. *p=0.0106 (day 7). ( D ) Quantification of clonogenicity in 2D and 3D from single CAL-148 shRNA SOX11 cells plated in presence or absence of DOX after 21 days. The number in brackets represents the mean in each group of the three experimental replicates. DOX: doxycycline, NS: non-silencing.

SOX11 Antibody in Western Blot (WB) — Figure 5. SOX11+ DCIS cells isolated from brain metastasis display a colonisation and growth advantage after intracranial xenografting. ( A ) Western blot of SOX11, MEX3A and TUBB3 in total cell lysates of EV and SOX11 cell lines isolated from primary metastasis at indicated sites in presence or absence of DOX. ( B ) Representative in vivo IVIS imaging 7 days after tail vein injections of iSOX11 cells that were isolated from the brain metastasis (SOX11Br) in presence or absence of DOX. ( C ) Tabulated results of micrometastasis from in vivo IVIS imaging 7 days after tail vein injections of SOX11Br cells. ( D ) Tabulated results of micrometastasis from ex vivo IVIS imaging of the tail vein injections of SOX11Br cells. ( E ) IVIS imaging of mice fed normal chow or DOX-containing chow 10 days after intracranial injections of SOX11Br cells. ( F ) Survival curve for mice shown in E. *p = 0.0195. DOX: doxycycline.

产品信息

13-9773-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

-

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免疫组化（石蜡） (IHC (P))

10 µg/mL

-

产品规格

种属反应

Human

已发表种属

Not Applicable

宿主/亚型

Mouse / IgG1, kappa

推荐的同型对照

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Biotin, eBioscience™

分类

Monoclonal

类型

Antibody

克隆号

SOX11-C1

偶联物

Biotin Biotin Biotin

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2572837

产品详细信息

Description: The monoclonal antibody SOX11-C1 (C1) recognizes human Sox11. The transcription factor Sox11 is a member of the SOX (sex determining region Y-related HMG (High Mobility Group) Box) family of proteins. Under normal conditions Sox11 is expressed in the developing central nervous system during embryogenesis and has sequence homology to Sox4. Sox11 plays a role in neuronal maturation and epithelial-mesenchymal interactions and is required for the survival of neuronal and mesenchymal progenitor cells. Sox11 is expressed in mantle cell lymphoma (MCL) and in subsets of hairy cell leukemias, Burkitt lymphomas, and B cell lymphoblastic leukemias, but is not expressed in other B cell lymphomas or in normal B lymphocytes. Sox11 is also expressed in epithelial ovarian cancer and gliomas.

Applications Reported: This SOX11-C1 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.

Applications Tested: This SOX11-C1 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded tissue using high pH antigen retrieval and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

This intronless gene encodes a member of the SOX family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. The protein may function in the developing nervous system and play a role in tumorigenesis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

SOX11 promotes epithelial/mesenchymal hybrid state and alters tropism of invasive breast cancer cells.

eLife

Oliemuller E,Newman R,Tsang SM,Foo S,Muirhead G,Noor F,Haider S,Aurrekoetxea-Rodríguez I,Vivanco MD,Howard BA

Published figure using SOX11 monoclonal antibody (Product # 13-9773-82) in Western Blot

Thu Sep 10 00:00:00 EDT 2020

生物信息学

蛋白别名: SRY; SRY (sex-determining region Y)-box 11; SRY-related HMG-box gene 11; Transcription factor SOX-11

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基因别名: MRD27; SOX11

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UniProt ID: (Human) P35716

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Entrez Gene ID: (Human) 6664

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transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription coactivator activity enhancer sequence-specific DNA binding transcription factor activity, sequence-specific DNA binding translation factor activity, RNA binding HMG box transcription factor

参与通路

negative regulation of transcription from RNA polymerase II promoter skeletal system development kidney development neural tube formation lens morphogenesis in camera-type eye outflow tract morphogenesis cardiac ventricle formation noradrenergic neuron differentiation transcription from RNA polymerase II promoter translation positive regulation of cell proliferation positive regulation of gene expression negative regulation of gene expression oligodendrocyte development glial cell proliferation neural crest cell development regulation of transforming growth factor beta receptor signaling pathway spinal cord development glial cell development neuron differentiation positive regulation of BMP signaling pathway positive regulation of hippo signaling skeletal muscle cell differentiation positive regulation of neuron differentiation positive regulation of osteoblast differentiation positive regulation of ossification positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of hormone secretion sympathetic nervous system development embryonic digestive tract morphogenesis embryonic skeletal system morphogenesis negative regulation of lymphocyte proliferation positive regulation of neurogenesis hard palate development soft palate development limb bud formation negative regulation of glial cell proliferation ventricular septum morphogenesis lung morphogenesis negative regulation of cell death neuroepithelial cell differentiation eyelid development in camera-type eye somite development cornea development in camera-type eye closure of optic fissure signal transduction involved in cell cycle checkpoint positive regulation of stem cell proliferation negative regulation of transcription regulatory region DNA binding positive regulation of lens epithelial cell proliferation