Invitrogen

SOX11 Monoclonal Antibody (SOX11-C1), eFluor™ 660, eBioscience™

SOX11 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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SOX11 Antibody (50-9773-82) in IHC (P)

Immunohistochemistry of formalin-fixed paraffin embedded human Mantle Cell lymphoma using 10 µg/mL of Mouse IgG1 K Isotype Control eFluor® 660 (left) or 10 µg/mL of Anti-Human Sox11 eFluor® 660 (right). Nuclei are stained with DAPI (colocalization appears pink).

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产品信息

50-9773-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

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免疫组化（石蜡） (IHC (P))

10 µg/mL

免疫细胞化学 (ICC/IF)

Assay-Dependent

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流式细胞分析 (Flow)

Assay-Dependent

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

SOX11-C1

偶联物

eFluor™ 660 eFluor™ 660 eFluor™ 660

激发/发射光谱

651/668 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2574372

产品详细信息

Description: The monoclonal antibody SOX11-C1 (C1) recognizes human Sox11. The transcription factor Sox11 is a member of the SOX (sex determining region Y-related HMG (High Mobility Group) Box) family of proteins. Under normal conditions Sox11 is expressed in the developing central nervous system during embryogenesis and has sequence homology to Sox4. Sox11 plays a role in neuronal maturation and epithelial-mesenchymal interactions and is required for the survival of neuronal and mesenchymal progenitor cells. Sox11 is expressed in mantle cell lymphoma (MCL) and in subsets of hairy cell leukemias, Burkitt lymphomas, and B cell lymphoblastic leukemias, but is not expressed in other B cell lymphomas or in normal B lymphocytes. Sox11 is also expressed in epithelial ovarian cancer and gliomas.

Applications Reported: This SOX11-C1 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, immunocytochemistry and flow cytometric analysis.

Applications Tested: This SOX11-C1 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low or high pH antigen retrieval and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interst.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

This intronless gene encodes a member of the SOX family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. The protein may function in the developing nervous system and play a role in tumorigenesis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

SOX11 promotes epithelial/mesenchymal hybrid state and alters tropism of invasive breast cancer cells.

eLife

Oliemuller E,Newman R,Tsang SM,Foo S,Muirhead G,Noor F,Haider S,Aurrekoetxea-Rodríguez I,Vivanco MD,Howard BA

50-9773 was used in Immunocytochemistry-immunoflourescence to find that that SOX11+DCIS tumour cells metastasize to brain and bone at greater frequency and to lungs at lower frequency compared to cells with lower SOX11 levels.