Invitrogen

SP4 Polyclonal Antibody

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SP4 Antibody (PA5-95764) in WB

Western blot analysis of SP4 in extracts of various cell lines. Samples were incubated with SP4 Polyclonal antibody (Product # PA5-95764) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

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产品信息

PA5-95764

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 280-440 of human SP4 (NP_0031032)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2.5 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.01% thimerosal

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2807566

产品详细信息

Immunogen sequence: TGQVGQPAAT ADSGTSNGNQ LVSTPTNTTT SASTMPESPS SSTTCTTTAS TSLTSSDTLV SSADTGQYAS TSASSSERTI EESQTPAATE SEAQSSSQLQ PNGMQNAQDQ SNSLQQVQIV GQPILQQIQI QQPQQQIIQA IPPQSFQLQS GQTIQTIQQQ P; Positive Samples: Mouse thymus, Mouse spleen, Mouse pancreas, Rat thymus; Cellular Location: Nucleus

靶标信息

This gene belongs to a family of Sp1 related genes that encode transcription factors that regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses the transcription of numerous genes. Transcript variants encoding different isoforms have been described for this gene, and one has been reported to initiate translation from a non-AUG (AUA) start codon. Additional isoforms, resulting from the use of alternate downstream translation initiation sites, have also been noted. A related pseudogene has been identified on chromosome 13.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。