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Application
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SPARCL1 Antibody (PA5-80063) in IHC (P)
Immunohistochemistry analysis of SPARCL1 on paraffin-embedded human colon cancer tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with SPARCL1 polyclonal antibody (Product# PA5-80063) with a dilution of 2 µg/mL (overnight at 4°C), followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). D... View More
Product Details
PA5-80063
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence of human SPARCL1 (ITRFFEECDPNKDKHITLKEWGHCFGIKEEDIDENLLF).
Conjugate
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2747178
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human U-87MG whole cell, human SHG-44 whole cell, human MDA-MB-231 whole cell, human K562 whole cell, rat C6 whole cell, mouse smooth muscle tissue. IHC: human colon cancer tissue, mouse brain tissue.
Target Information
This is a secreted protein. Found in the extracellular matrix. It's highly expressed in lymph node, brain, heart, lung, skeletal muscle, ovary, small intestine, and colon, with lower levels in placenta, pancreas, testis, spleen, and thymus, and no expression in kidney, liver, and peripheral blood leukocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Extracellular matrix protein 2; Hevin; High endothelial venule protein; MAST 9; Matrix glycoprotein Sc1; proliferation-inducing protein 33; SPARC-like 1 (hevin); SPARC-like 1 (mast9, hevin); SPARC-like protein 1
Gene Aliases: Ecm2; hevin; MAST 9; MAST9; PIG33; SC1; SPARCL1
UniProt ID: (Human) E7ESU2, (Mouse) P70663, (Rat) P24054
Entrez Gene ID: (Human) 8404, (Mouse) 13602, (Rat) 25434
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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