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SPIRE2 Antibody (PA5-120464) in WB
Western blot analysis of SPIRE2 in extracts of various cells. Samples were incubated with SPIRE2 Polyclonal antibody (Product # PA5-120464) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
产品信息
PA5-120464
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human SPIRE2 (NP_115827.1).
偶联物
形式
Liquid
浓度
0.38 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2914036
产品详细信息
Positive test controls include: U-87MG, A431, HeLa, mouse liver, mouse brain, mouse testis, rat brain, rat testis. The target is usually found in the following locations: Cell membrane, Cytoplasm, Cytoplasmic side, Cytoplasmic vesicle membrane, Peripheral membrane protein, cytoskeleton, cytosol, perinuclear region.
Immunogen sequence: MARAGSCGGA AAGAGRPEPW ELSLEEVLKA YEQPLNEEQA WAVCFQGCRG LRGSPGRRLR DTGDLLLRGD GSVGAREPEA AEPATMVVPL ASSEAQTVQS LGFAIYRALD WGLDESEERE LSPQLERLID LMANNDSEDS GCGAADEGYG GPEEEEEAEG VPRSVRTFAQ AMRLCAARLT DPRGAQAHYQ
靶标信息
Acts as an actin nucleation factor, remains associated with the slow-growing pointed end of the new filament (PubMed:21620703). Involved in intracellular vesicle transport along actin fibers, providing a novel link between actin cytoskeleton dynamics and intracellular transport. Required for asymmetric spindle positioning and asymmetric cell division during meiosis (PubMed:21620703). Required for normal formation of the cleavage furrow and for polar body extrusion during female germ cell meiosis (PubMed:21620703). Also acts in the nucleus: together with SPIRE1 and SPIRE2, promotes assembly of nuclear actin filaments in response to DNA damage in order to facilitate movement of chromatin and repair factors after DNA damage (PubMed:26287480). [UniProt]
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Protein spire homolog 2; Spir-2; spire actin nucleation factor 2; spire family actin nucleation factor 2; spire homolog 2; spire-type actin nucleation factor 2
基因别名: BC026502; KIAA1832; Spir-2; SPIR2; SPIRE2
UniProt ID: (Human) Q8WWL2, (Mouse) Q8K1S6
Entrez Gene ID: (Human) 84501, (Rat) 307925, (Mouse) 234857
