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SPTLC1 Antibody (PA5-115960) in ICC/IF
Immunocytochemistry analysis of SPTLC1 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115960) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
产品信息
PA5-115960
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human SPTLC1(Accession O15269), corresponding to amino acid residues S313-F363.
偶联物
Unconjugated
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2900594
产品详细信息
Antibody detects endogenous levels of total SPTLC1.
靶标信息
Serine palmitoyltransferase (SPT) is the key enzyme in sphingolipid biosynthesis. It catalyzes the pyridoxal-5-prime-phosphate-dependent condensation of L-serine and palmitoyl-CoA to 3-oxosphinganine.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: hLCB1; HSAN; LCB 1; Long chain base biosynthesis protein 1; MGC14645; RP11-118F2.1; serine C-palmitoyltransferase; Serine palmitoyltransferase 1; serine palmitoyltransferase subunit 1; serine palmitoyltransferase, long chain base subunit 1; Serine-palmitoyl-CoA transferase 1; SPT 1; SPT1
基因别名: AW552086; C77762; E030036H05; HSAN1; HSN1; LBC1; LCB1; rCG_44191; RGD1306617; SPT1; SPTI; SPTLC1
UniProt ID: (Human) O15269, (Mouse) O35704, (Rat) D4A2H2
Entrez Gene ID: (Human) 10558, (Mouse) 268656, (Rat) 361213
