Invitrogen

SQSTM1 Polyclonal Antibody

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

2个已发表图片

5 篇参考文献

查看其他49个SQSTM1抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

SQSTM1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 13

SQSTM1 Antibody (PA5-27247) in ICC/IF

SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at autophagosome by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: SQSTM1 protein stained by SQSTM1 antibody [N3C1], Internal (Product # PA5-27247) diluted at 1:1,000. Red: Phalloidin, a F-actin marker.

SQSTM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SQSTM1 Antibody in Flow Cytometry (Flow)

SQSTM1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

SQSTM1 Antibody in Immunohistochemistry (IHC)

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产品信息

PA5-27247

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

查看3篇已发表文章 3篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

1:50-1:200

免疫沉淀 (IP)

1:100-1:500

产品规格

种属反应

Bovine, Human, Insect, Mouse, Rat

已发表种属

Human, Mouse, Non-human primate

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to a region within amino acids 157 and 251 of Human SQSTM1

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.39 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol, 1% BSA

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2544723

产品详细信息

Recommended positive controls: A549, HepG2, HepG2 (3µM Thapsigargin treatment for 24 hr), Huh7, Huh7 ( 3µM Thapsigargin, 12hr), NIH-3T3, JC, BCL-1, PC-12, Rat2, HeLa.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Protein Arginylation Is Regulated during SARS-CoV-2 Infection.

Viruses

Macedo-da-Silva J,Rosa-Fernandes L,Gomes VM,Santiago VF,Santos DM,Molnar CMS,Barboza BR,de Souza EE,Marques RF,Boscardin SB,Durigon EL,Marinho CRF,Wrenger C,Marie SKN,Palmisano G

PA5-27247 was used in Western Blotting to demonstrate that ATE1 is increased during SARS-CoV-2 infection and its inhibition has potential therapeutic value.

Thu Jan 19 00:00:00 EST 2023

Protein Arginylation Is Regulated during SARS-CoV-2 Infection.

Viruses

Macedo-da-Silva J,Rosa-Fernandes L,Gomes VM,Santiago VF,Santos DM,Molnar CMS,Barboza BR,de Souza EE,Marques RF,Boscardin SB,Durigon EL,Marinho CRF,Wrenger C,Marie SKN,Palmisano G

PA5-27247 was used in Western Blotting to demonstrate that ATE1 is increased during SARS-CoV-2 infection and its inhibition has potential therapeutic value.

Thu Jan 19 00:00:00 EST 2023

Protein Arginylation Is Regulated during SARS-CoV-2 Infection.

Viruses

Macedo-da-Silva J,Rosa-Fernandes L,Gomes VM,Santiago VF,Santos DM,Molnar CMS,Barboza BR,de Souza EE,Marques RF,Boscardin SB,Durigon EL,Marinho CRF,Wrenger C,Marie SKN,Palmisano G

PA5-27247 was used in Western Blotting to demonstrate that ATE1 is increased during SARS-CoV-2 infection and its inhibition has potential therapeutic value.

Thu Jan 19 00:00:00 EST 2023

Synergic Effect of Metformin and Everolimus on Mitochondrial Dynamics of Renal Cell Carcinoma.

Genes

Hong SH,Lee KS,Hwang HJ,Park SY,Han WK,Yoon YE

Published figure using SQSTM1 polyclonal antibody (Product # PA5-27247) in Immunohistochemistry

Wed Jul 06 00:00:00 EDT 2022

Tau Oligomers and Fibrils Exhibit Differential Patterns of Seeding and Association With RNA Binding Proteins.

Frontiers in neurology

Jiang L,Zhao J,Cheng JX,Wolozin B

PA5-27247 was used in Immunocytochemistry-immunoflourescence to explore the actions of two distinct types of tau aggregates, tau oligomers and tau fibrils in primary neuron cultures expressing human 4R0N tau.

Fri Jun 04 00:00:00 EDT 2021

Tau Oligomers and Fibrils Exhibit Differential Patterns of Seeding and Association With RNA Binding Proteins.

Frontiers in neurology

Jiang L,Zhao J,Cheng JX,Wolozin B

Fri Jun 04 00:00:00 EDT 2021

Tau Oligomers and Fibrils Exhibit Differential Patterns of Seeding and Association With RNA Binding Proteins.

Frontiers in neurology

Jiang L,Zhao J,Cheng JX,Wolozin B

Fri Jun 04 00:00:00 EDT 2021

Allele-selective lowering of mutant HTT protein by HTT-LC3 linker compounds.

Nature

Li Z,Wang C,Wang Z,Zhu C,Li J,Sha T,Ma L,Gao C,Yang Y,Sun Y,Wang J,Sun X,Lu C,Difiglia M,Mei Y,Ding C,Luo S,Dang Y,Ding Y,Fei Y,Lu B

PA5-27247 was used in Western Blotting to demonstrate the concept of lowering levels of disease-causing proteins using autophagosome-tethering compounds.

Fri Nov 01 00:00:00 EDT 2019

生物信息学

蛋白别名: EBI3-associated protein of 60 kDa; EBI3-associated protein p60; EBIAP; oxidative stress induced; oxidative stress induced like; phosphotyrosine independent ligand for the Lck SH2 domain p62; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; PKC-zeta-interacting protein; Protein kinase C-zeta-interacting protein; Sequestosome-1; STONE14; Ubiquitin-binding protein p62

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基因别名: A170; FTDALS3; ORCA; OSF-6; Osi; OSIL; p60; p62; p62B; PDB3; SQSTM1; STAP; STONE14; ZIP; ZIP3

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UniProt ID: (Human) Q13501, (Rat) O08623, (Mouse) Q64337

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Entrez Gene ID: (Bovine) 338045, (Human) 8878, (Rat) 113894, (Mouse) 18412

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