Invitrogen

SREBP1 Polyclonal Antibody

SREBP1 Antibody in Immunocytochemistry (ICC/IF)

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SREBP1 Antibody (PA1-337) in ICC/IF

Immunofluorescence analysis of SREBF1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SREBP1 Polyclonal Antibody (Product # PA1-337, 1:200) in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A32790, 1:2,000), ... View More

SREBP1 Antibody in Immunohistochemistry (IHC)

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产品信息

PA1-337

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:5,000

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免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:50-1:500

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to residues M(32) L Q L I N N Q D S D F P G L F(47) of mouse SREBP 1.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2194231

产品详细信息

PA1-337 detects SREBP 1 in mouse and rat samples.

PA1-337 has been successfully used in Western blot and ICC/IF procedures. By Western blot, this antibody detects an ~68 and 120 kDa protein representing SREBP 1 in mouse and rat liver samples as well as rat kidney samples. A predominent band at ~68 kDa (active cleaved site) is seen and a band at ~120 kDa (inactive precursor) may not be seen or it may be diminished.

The PA1-337 immunogen is a synthetic peptide corresponding to residues M(32) L Q L I N N Q D S D F P G L F(47) of mouse SREBP 1. This sequence is identical in human and rat. PA1-337 immunizing peptide (Cat. # PEP-274) is available for use in neutralization and control experiments.

靶标信息

Sterol regulatory element-binding proteins (SREBPs) are transcription factors that are members of the basic helix-loop-helix leucine zipper family of DNA binding proteins. Three isoforms have been identified in mammalian tissues that vary in structure, regulation, and function. SREBP-1a and SREBP-1c (originally cloned as ADD1) are protein products of alternative promoter usage of the SREBP-1 gene. The third isoform is transcribed from a different gene, SREBP-2. SREBPs are present as 120 kDa inactive precursors in the endoplasmic reticulum (ER) membrane. Upon activation, the SREBP protein is translocated to the Golgi and proteolytic cleavage occurs resulting in a mature transcriptionally active 60-78 kDa fragment. In liver and adipose tissues, SREBPs have a significant influence on lipid and cholesterol accumulation by inducing the transcription of genes involved in these processes. While SREBP-1 is thought to be more important in regulating the expression of genes involved in triglyceride synthesis and accumulation, SREBP-2 has been more closely linked to those involved in cholesterol synthesis and accumulation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

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Rungsa P,San HT,Sritularak B,Böttcher C,Prompetchara E,Chaotham C,Likhitwitayawuid K

Published figure using SREBP1 polyclonal antibody (Product # PA1-337) in Western Blot

Mon Feb 27 00:00:00 EST 2023

BCATc inhibitor 2 ameliorated mitochondrial dysfunction and apoptosis in oleic acid-induced non-alcoholic fatty liver disease model.

Frontiers in pharmacology

Lu Z,Sun GF,Pan XA,Qu XH,Yang P,Chen ZP,Han XJ,Wang T

Published figure using SREBP1 polyclonal antibody (Product # PA1-337) in Western Blot

Fri Nov 18 00:00:00 EST 2022

A Botanical Mixture Consisting of Inula japonica and Potentilla chinensis Relieves Obesity via the AMPK Signaling Pathway in 3T3-L1 Adipocytes and HFD-Fed Obese Mice.

Nutrients

Lee SY,Chung KS,Son SR,Lee SY,Jang DS,Lee JK,Kim HJ,Na CS,Lee SH,Lee KT

PA1-337 was used in Western Blotting to indicate that EEIP improves HFD-induced obesity through adipogenesis inhibition in the WAT and liver and is a promising dietary natural material for improving obesity.

Tue Sep 06 00:00:00 EDT 2022

Keratin 17 Is Required for Lipid Metabolism in Keratinocytes and Benefits Epidermal Permeability Barrier Homeostasis.

Frontiers in cell and developmental biology

Pang B,Zhu Z,Xiao C,Luo Y,Fang H,Bai Y,Sun Z,Ma J,Dang E,Wang G

PA1-337 was used in Western Blot to demonstrate that K17 contributes to the restoration of the epidermal permeability barrier via stabilizing lipid metabolism in keratinocytes.

Tue Feb 01 00:00:00 EST 2022

Keratin 17 Is Required for Lipid Metabolism in Keratinocytes and Benefits Epidermal Permeability Barrier Homeostasis.

Frontiers in cell and developmental biology

Pang B,Zhu Z,Xiao C,Luo Y,Fang H,Bai Y,Sun Z,Ma J,Dang E,Wang G

PA1-337 was used in Western Blot to demonstrate that K17 contributes to the restoration of the epidermal permeability barrier via stabilizing lipid metabolism in keratinocytes.

Tue Feb 01 00:00:00 EST 2022

Gycyrrhizic acid alleviates atherosclerotic lesions in rats with diabetes mellitus.

Molecular medicine reports

Zhao Y,Li W,Zhang D

Published figure using SREBP1 polyclonal antibody (Product # PA1-337) in Western Blot

Mon Nov 01 00:00:00 EDT 2021

Polygonatum sibiricum F. Delaroche polysaccharide ameliorates HFD‑induced mouse obesity via regulation of lipid metabolism and inflammatory response.

Molecular medicine reports

Liu B,Tang Y,Song Z,Ge J

PA1-337 was used in Western Blotting to suggest that PSP exhibited lipid‚lowering and anti‚inflammatory effects by activating the AMPK signaling pathway.

Thu Jul 01 00:00:00 EDT 2021

Polygonatum sibiricum F. Delaroche polysaccharide ameliorates HFD‑induced mouse obesity via regulation of lipid metabolism and inflammatory response.

Molecular medicine reports

Liu B,Tang Y,Song Z,Ge J

PA1-337 was used in Western Blotting to suggest that PSP exhibited lipid‚lowering and anti‚inflammatory effects by activating the AMPK signaling pathway.

Thu Jul 01 00:00:00 EDT 2021

Long term N-acetylcysteine administration rescues liver steatosis via endoplasmic reticulum stress with unfolded protein response in mice.

Lipids in health and disease

Tsai CC,Chen YJ,Yu HR,Huang LT,Tain YL,Lin IC,Sheen JM,Wang PW,Tiao MM

PA1-337 was used in Western Blotting to examine the mechanisms of liver steatosis after 12 months of high fat diet and tested the ability of N-acetylcysteine to rescue liver steatosis.

Mon May 25 00:00:00 EDT 2020

Long term N-acetylcysteine administration rescues liver steatosis via endoplasmic reticulum stress with unfolded protein response in mice.

Lipids in health and disease

Tsai CC,Chen YJ,Yu HR,Huang LT,Tain YL,Lin IC,Sheen JM,Wang PW,Tiao MM

PA1-337 was used in Western Blotting to examine the mechanisms of liver steatosis after 12 months of high fat diet and tested the ability of N-acetylcysteine to rescue liver steatosis.

Mon May 25 00:00:00 EDT 2020

Liver X Receptor activation regulates genes involved in lipid homeostasis in developing chondrocytes.

Osteoarthritis and cartilage open

Sun MM,Beier F

PA1-337 was used in Immunocytochemistry, Western Blot, Immunohistochemistry to show that LXR activation alters expression of lipid metabolism genes in growth plate chondrocytes, in part through activation of molecules responsible for cellular cholesterol efflux.

Sun Mar 01 00:00:00 EST 2020

Liver X Receptor activation regulates genes involved in lipid homeostasis in developing chondrocytes.

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Sun MM,Beier F

Sun Mar 01 00:00:00 EST 2020

Sterol regulatory element binding protein and dietary lipid regulation of fatty acid synthesis in the mammary epithelium.

American journal of physiology. Endocrinology and metabolism

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PA1-337 was used in western blot to investigate two modes of regulation about fatty acid synthesis in the mammary gland of lactating mice

Wed Dec 01 00:00:00 EST 2010

生物信息学

蛋白别名: ADD1; Adipocyte determination- and differentiation-dependent factor 1; bHLHd1; Class D basic helix-loop-helix protein 1; nt-SREBP1; SREBP-1; Sterol regulatory element-binding protein 1; Sterol regulatory element-binding transcription factor 1

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基因别名: ADD-1; ADD1; BHLHD1; D630008H06; SREBF1; SREBP-1; SREBP-1a; SREBP-1c; SREBP1; SREBP1a; SREBP1c

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UniProt ID: (Human) P36956, (Rat) P56720, (Mouse) Q9WTN3

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Entrez Gene ID: (Human) 6720, (Rat) 78968, (Mouse) 20787

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功能