Invitrogen

SREBP2 Polyclonal Antibody

SREBP2 Antibody in Immunocytochemistry (ICC/IF)

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SREBP2 Antibody (PA1-338) in ICC/IF

Immunofluorescent analysis of SREBP2 (green) showing staining in the cytoplasm of PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a SREBP2 polyclonal antibody (Product # PA1-338) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin... View More

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产品信息

PA1-338

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:100-1:1,000

查看7篇已发表文章 7篇已发表文章

免疫细胞化学 (ICC/IF)

1:20-1:200

流式细胞分析 (Flow)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to residues A(843) I S W L Q G D D A A V R S H(857) of rat SREBP 2.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2194237

产品详细信息

PA1-338 detects SREBP 2 in human, mouse and rat samples.

The PA1-338 immunogen is a synthetic peptide corresponding to residues A(843) I S W L Q G D D A A V R S H(857) of rat SREBP 2. This sequence is identical in human and differs by 1 a.a. in mouse. PA1-338 immunizing peptide (Cat. # PEP-275) is available for use in neutralization and control experiments.

靶标信息

This gene encodes a ubiquitously expressed transcription factor that controls cholesterol homeostasis by stimulating transcription of sterol-regulated genes. The encoded protein contains a basic helix-loop-helix-leucine zipper (bHLH-Zip) domain.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

TFEB inhibition induces melanoma shut-down by blocking the cell cycle and rewiring metabolism.

Cell death & disease

Ariano C,Costanza F,Akman M,Riganti C,Corà D,Casanova E,Astanina E,Comunanza V,Bussolino F,Doronzo G

PA1-338 was used in Western Blot to suggest that the oncogenic transcription factor EB (TFEB), a key regulator of lysosomal biogenesis and function, controls melanoma tumour growth through a transcriptional programme targeting ERK1/2 activity and glucose, glutamine and cholesterol metabolism.

Tue May 09 00:00:00 EDT 2023

N¹-methyladenosine methylation in tRNA drives liver tumourigenesis by regulating cholesterol metabolism.

Nature communications

Wang Y,Wang J,Li X,Xiong X,Wang J,Zhou Z,Zhu X,Gu Y,Dominissini D,He L,Tian Y,Yi C,Fan Z

PA1-338 was used in Western Blot to show that N1-methyladenosine methylation (m1A) in tRNA is remarkably elevated in hepatocellular carcinoma (HCC) patient tumour tissues.

Tue Nov 02 00:00:00 EDT 2021

N¹-methyladenosine methylation in tRNA drives liver tumourigenesis by regulating cholesterol metabolism.

Nature communications

Wang Y,Wang J,Li X,Xiong X,Wang J,Zhou Z,Zhu X,Gu Y,Dominissini D,He L,Tian Y,Yi C,Fan Z

PA1-338 was used in Western Blot to show that N1-methyladenosine methylation (m1A) in tRNA is remarkably elevated in hepatocellular carcinoma (HCC) patient tumour tissues.

Tue Nov 02 00:00:00 EDT 2021

The cholesterol metabolite 25-hydroxycholesterol restrains the transcriptional regulator SREBP2 and limits intestinal IgA plasma cell differentiation.

Immunity

Trindade BC,Ceglia S,Berthelette A,Raso F,Howley K,Muppidi JR,Reboldi A

PA1-338 was used in Immunocytochemistry, Western Blot, Flow Cytometry to examine how the cholesterol metabolite 25-hydroxycholesterol (25-HC) impacts the intestinal B cell response.

Tue Oct 12 00:00:00 EDT 2021

Autophagy regulates fatty acid availability for oxidative phosphorylation through mitochondria-endoplasmic reticulum contact sites.

Nature communications

Bosc C,Broin N,Fanjul M,Saland E,Farge T,Courdy C,Batut A,Masoud R,Larrue C,Skuli S,Espagnolle N,Pagès JC,Carrier A,Bost F,Bertrand-Michel J,Tamburini J,Récher C,Bertoli S,Mansat-De Mas V,Manenti S,Sarry JE,Joffre C

PA1-338 was used in Western Blot to establish that mitochondria, through the regulation of MERCs, controls autophagy that, in turn finely tunes lipid degradation to fuel OxPHOS supporting proliferation and growth in leukemia.

Thu Aug 13 00:00:00 EDT 2020

p53 Represses the Mevalonate Pathway to Mediate Tumor Suppression.

Cell

Moon SH,Huang CH,Houlihan SL,Regunath K,Freed-Pastor WA,Morris JP,Tschaharganeh DF,Kastenhuber ER,Barsotti AM,Culp-Hill R,Xue W,Ho YJ,Baslan T,Li X,Mayle A,de Stanchina E,Zender L,Tong DR,D'Alessandro A,Lowe SW,Prives C

PA1-338 was used in Western Blotting to demonstrate that repression of the mevalonate pathway is a crucial component of p53-mediated liver tumor suppression and report on the mechanisms involved in this.

Thu Jan 24 00:00:00 EST 2019

Absence of gravin-mediated signaling inhibits development of high-fat diet-induced hyperlipidemia and atherosclerosis.

American journal of physiology. Heart and circulatory physiology

Fan Q,Yin X,Rababa'h A,Diaz Diaz A,Wijaya CS,Singh S,Suryavanshi SV,Vo HH,Saeed M,Zhang Y,McConnell BK

PA1-338 was used in Western Blotting to investigate the role of gravin in the development of high-fat diet (HFD)-induced atherosclerosis and hyperlipidemia.

Tue Oct 01 00:00:00 EDT 2019

Pleiotropic Role of p53 in Injury and Liver Regeneration after Acetaminophen Overdose.

The American journal of pathology

Borude P,Bhushan B,Gunewardena S,Akakpo J,Jaeschke H,Apte U

PA1-338 was used in Western Blotting to investigate the role of p53 in liver injury and regeneration after acetaminophen overdose.

Fri Jun 01 00:00:00 EDT 2018

High copper selectively alters lipid metabolism and cell cycle machinery in the mouse model of Wilson disease.

The Journal of biological chemistry

Huster D,Purnat TD,Burkhead JL,Ralle M,Fiehn O,Stuckert F,Olson NE,Teupser D,Lutsenko S

PA1-338 was used in western blot to investigate the role of SREBP2 during the copper-induced alteration of lipid metabolism and cell cycle machinery.

Fri Mar 16 00:00:00 EDT 2007

生物信息学

蛋白别名: bHLHd2; Class D basic helix-loop-helix protein 2; srebf2; srebf2 {ECO:0000312|EMBL:AAI01903.1}; SREBP-2; sterol regulatory element binding factor 2; sterol regulatory element binding protein 2; Sterol regulatory element-binding protein 2; Sterol regulatory element-binding transcription factor 2

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基因别名: AI608257; BHLHD2; lop13; nuc; SREBF2; Srebf2_retired; SREBP-2; SREBP2; SREBP2gc

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UniProt ID: (Human) Q12772, (Rat) Q3T1I5, (Mouse) Q3U1N2

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Entrez Gene ID: (Human) 6721, (Rat) 300095, (Mouse) 20788

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功能