Invitrogen

SSEA1 Monoclonal Antibody (MC-480), DyLight™ 550

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

查看其他65个SSEA1 (CD15)抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (2)
高级验证 (1)

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SSEA1 Antibody (MA1-022-D550) in Flow

THP-1 and Ramos cells were fixed and permeabilized using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-000) and then stained with SSEA1 Monoclonal Antibody (MC-480), DyLight™ 550 (Product # MA1-022-D550) (yellow histogram) at 1:100 dilution and CD19 Monoclonal Antibody (HIB19), APC, eBioscience™ (Product # 17-0199-42) (red histogram) at a concentration of 1 µg or left unstained (black histogram). CD15 and CD19 were mutually exclusively present on THP-1 and Ramos cells respectively. Viable cells, as determined ... View More

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SSEA1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of CD15 was observed in THP-1 in comparison to low to negative in Ramos using SSEA1 Monoclonal Antibody (MC-480), DyLight™ 550 (Product # MA1-022-D550). {RE}

产品信息

MA1-022-D550

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

1:50-1:500

产品规格

种属反应

Human, Mouse

宿主/亚型

Mouse / IgM

分类

Monoclonal

类型

Antibody

克隆号

MC-480

抗原

BALB/c mouse immunized with F9 teratocarcinoma stem cells (X-irradiated).

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偶联物

DyLight™ 550 DyLight™ 550 DyLight™ 550

激发/发射光谱

556/570 nm 查看光谱

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography - MBP

保存液

PBS with proprietary stabilizer

内含物

0.02% sodium azide

保存条件

4° C, do not freeze

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2536695

产品详细信息

MA1-022-D550 has been successfully used in flow cytometry on mouse and human samples.

靶标信息

CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: 3-fucosyl-N-acetyl-lactosamine epitope; 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase; alpha (1,3) fucosyltransferase, myeloid specific; alpha (1,3) fucosyltransferase, myeloid-specific; alpha 1,3-fucosyltransferase; Alpha-(1,3)-fucosyltransferase 4; ELAM ligand fucosyltransferase; ELAM-1 ligand fucosyltransferase; Fuc-TIV; Fucosyltransferase 4; fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific); Fucosyltransferase IV; fucT-IV; FUTIV; Galactoside 3-L-fucosyltransferase; Lewis X; SSEA-1; stage-specific embryonic antigen 1

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基因别名: AI451562; CD15; ELFT; FAL; FCT3A; FUC-TIV; FucT-IV; FUT4; FUTIV; LeX; SSEA-1; Ssea1

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UniProt ID: (Human) P22083, (Mouse) Q11127

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Entrez Gene ID: (Human) 2526, (Mouse) 14345

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