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SSEA3 Antibody (MA1-020-D650) in ICC/IF
Immunofluorescent analysis of SSEA-3 (red) showing membrane staining of NCCIT cells (right panel) compared to a negative HeLa control (left panel). The cells were fixed with formalin for 15 minutes, washed, and then blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a DyLight 650-conjugated SSEA-3 monoclonal antibody (Product # MA1-020-D650) in 3% BSA-PBS at a dilution of 1:20 and incubated for 1 hour at 37C in the dark. Nuclei (blue) were stained with DAPI. Images were taken at 60X magnification.
产品信息
MA1-020-D650
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Rat
/ IgM
分类
Monoclonal
类型
Antibody
克隆号
MC-631
抗原
Fischer rat immunized with 4-8 cell stage mouse embryos
偶联物
激发/发射光谱
651/673 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography - MBP
保存液
PBS with proprietary stabilizer
内含物
0.02% sodium azide
保存条件
4° C, do not freeze
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2536684
产品详细信息
MA1-020-D650 has been successfully used in ICC/IF and flow cytometry applications on human samples.
靶标信息
SSEA-3 (stage-specific embryonic antigen 3) is a cell surface antigen, expressed along with SSEA-4, TRA-1-60 and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells, and induced pluripotent stem cells (iPS). In human cells, these surface markers are down-regulated during the differentiation process, while SSEA1 is absent in undifferentiated human stem cells and upregulated on the cell surface after retinoic acid mediated differentiation. Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: SSEA-3; stage-specific embryonic antigen 3
