Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 1
SSEA5 Antibody (MA1-144-D755) in ICC/IF
Immunofluorescent analysis of SSEA-5 in NCCIT (3 left bars) and negative control HeLa cells (3 right bars) in a 96 well plate assay. Formalin-fixed cells were blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a DyLight 755-conjugated SSEA-5 monoclonal antibody (Product # MA1-144-D755) in 1% BSA-PBS at a dilution of 1:100 for 1 hour at room temperature, washed with PBS, and fluorescence intensity was measured at 800nm using a near-IR imaging system. Fluorescence intensities from 3 indep... View More
产品信息
MA1-144-D755
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
8E11
抗原
Undifferentiated H9 human embryonic stem cells
偶联物
激发/发射光谱
760/789 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS with proprietary stabilizer
内含物
0.02% sodium azide
保存条件
4° C, do not freeze
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2536852
产品详细信息
Immunofluorescent analysis of MA1-144-D755 shows positive staining of SSEA-5 in human embryonal carcinoma NCCIT cells.
靶标信息
Stage-Specific Embryonic Antigen-5 (SSEA-5) is an oligosaccharide that can be part of glycosphingolipids and glycoproteins. It contains the motif Fuc1-2Gal-beta1-3GlcNAc-beta, which is characteristic of H-1 antigens. SSEA-5 is found in the inner cell mass of the human blastocyst during embryogenesis, on undifferentiated human embryonic stem cells and on the surface of induced pluripotent cells. SSEA-5 was found to proceed the loss of other known stem cell markers and the combination of SSEA-5 with either CD9 and CD90 or CD50 and CD200 was superior in identifying and eliminating rare undifferentiated human ES cells from differentiated cell populations than the use of other established stem cell markers.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: SSEA-5
