Bethyl Laboratories

STAT5b Recombinant Rabbit Monoclonal Antibody (BLR146J)

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抗体检测数据 (6)

STAT5b Antibody in Immunocytochemistry (ICC/IF)

STAT5b Antibody in Immunocytochemistry (ICC/IF)

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STAT5b Antibody (A700-146) in ICC/IF

Detection of human STAT5b in FFPE HDLM-2 cells by immunocytochemistry. Antibody: Rabbit anti-STAT5b recombinant monoclonal [BLR146H] (A700-146 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

STAT5b Antibody in Immunocytochemistry (ICC/IF)

STAT5b Antibody in Immunohistochemistry (Paraffin) (IHC (P))

STAT5b Antibody in Western Blot (WB)

STAT5b Antibody in Western Blot (WB)

STAT5b Antibody in Flow Cytometry (Flow)

STAT5b Antibody in Immunoprecipitation (IP)

Product Image Gallery

STAT5b Antibody in Immunocytochemistry (ICC/IF) — Detection of human STAT5b in FFPE HDLM-2 cells by immunocytochemistry. Antibody: Rabbit anti-STAT5b recombinant monoclonal [BLR146H] (A700-146 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

STAT5b Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Detection of human STAT5b in FFPE breast carcinoma by immunohistochemistry. Antibody: Rabbit anti-STAT5b recombinant monoclonal antibody [BLR146H] (A700-146 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

STAT5b Antibody in Western Blot (WB) — Detection of human STAT5b by western blot. Samples: Whole cell lysate (10 µg) from My-La CD4+, Hep-G2, HEK293T, Jurkat, and K-562 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-STAT5b recombinant monoclonal antibody [BLR146J] (A700-146 lot 1) used at 1:1,000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-COPB2 antibody (A304-523A).

STAT5b Antibody in Western Blot (WB) — Detection of mouse STAT5b by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-STAT5b recombinant monoclonal antibody [BLR146J] (A700-146 lot 1) used at 1:1,000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody (A304-523A).

STAT5b Antibody in Flow Cytometry (Flow) — Detection of human STAT5b (shaded) in Ramos cells by flow cytometry. Antibody: Rabbit anti-STAT5b recombinant monoclonal [BLR146J] (A700-146) or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-rabbit IgG (A120-101D2).

STAT5b Antibody in Immunoprecipitation (IP) — Detection of human STAT5b by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from My-La CD4+ cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-STAT5b recombinant monoclonal antibody [BLR146J] (A700-146 lot 1) used for IP at 6 µl per reaction. STAT5b was also immunoprecipitated by another rabbit anti-STAT5b antibody (A303-495A). For blotting immunoprecipitated STAT5b, A700-146 was used at 1:1,000. Detection: Chemiluminescence with an exposure time of 3 seconds.

产品信息

A700-146

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

-

免疫组化（石蜡） (IHC (P))

1:100-1:500

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

流式细胞分析 (Flow)

Assay-dependent

-

免疫沉淀 (IP)

6 µL/mg of lysate

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

Expression System

HEK293F

分类

Recombinant Monoclonal

类型

Antibody

克隆号

BLR146J

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

purified

保存液

BBS, pH 8.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Immunocytochemistry: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

靶标信息

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APLL). The dysregulation of the signaling pathways mediated by this protein may be the cause of the APLL.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: mammary gland factor STAT5B; Signal transducer and activator of transcription 5B; STA5B; STAT; transcription factor STAT5B

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基因别名: STAT5; STAT5B

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UniProt ID: (Human) P51692, (Mouse) Q541Q5

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Entrez Gene ID: (Human) 6777, (Mouse) 20851

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RNA polymerase II core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding chromatin binding transcription factor activity, sequence-specific DNA binding protein tyrosine kinase activity signal transducer activity protein binding protein phosphatase binding glucocorticoid receptor binding protein dimerization activity DNA binding double-stranded DNA binding sequence-specific DNA binding DNA-binding transcription factor

参与通路

allantoin metabolic process luteinization response to hypoxia natural killer cell differentiation liver development citrate metabolic process 2-oxoglutarate metabolic process succinate metabolic process oxaloacetate metabolic process regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter isoleucine metabolic process valine metabolic process creatine metabolic process fatty acid metabolic process acute-phase response JAK-STAT cascade female pregnancy lactation peptidyl-tyrosine phosphorylation regulation of steroid metabolic process taurine metabolic process lipid storage regulation of epithelial cell differentiation response to estradiol response to lipopolysaccharide positive regulation of natural killer cell proliferation positive regulation of natural killer cell differentiation cellular response to hormone stimulus T cell differentiation in thymus prolactin signaling pathway regulation of multicellular organism growth positive regulation of multicellular organism growth positive regulation of activated T cell proliferation progesterone metabolic process T cell homeostasis negative regulation of apoptotic process positive regulation of interleukin-2 biosynthetic process response to ethanol positive regulation of B cell differentiation positive regulation of gamma-delta T cell differentiation negative regulation of erythrocyte differentiation positive regulation of erythrocyte differentiation positive regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter positive regulation of natural killer cell mediated cytotoxicity creatinine metabolic process development of secondary female sexual characteristics development of secondary male sexual characteristics Peyer's patch development positive regulation of smooth muscle cell proliferation positive regulation of inflammatory response positive regulation of cellular component movement JAK-STAT cascade involved in growth hormone signaling pathway response to interleukin-2 response to interleukin-4 response to interleukin-15 cellular response to growth factor stimulus cellular response to epidermal growth factor stimulus mast cell migration transcription, DNA-templated regulation of transcription, DNA-templated signal transduction sex differentiation positive regulation of cell proliferation cytokine-mediated signaling pathway regulation of cell adhesion response to peptide hormone positive regulation of lymphocyte differentiation