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STT3B Antibody (PA5-106380) in IHC (P)
Immunohistochemistry analysis of STT3B in human pancreatic cancer and adjacent normal tissues. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with STT3B polyclonal antibody (Product # PA5-106380) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
产品信息
PA5-106380
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human STT3B(Accession Q8TCJ2), corresponding to amino acid residues C715-W765.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2854055
产品详细信息
Antibody detects endogenous levels of total STT3B.
靶标信息
Catalytic subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity. This subunit contains the active site and the acceptor peptide and donor lipid-linked oligosaccharide (LLO) binding pockets. STT3B is present in a small subset of OST complexes and mediates both cotranslational and post-translational N-glycosylation of target proteins: STT3B-containing complexes are required for efficient post-translational glycosylation and while they are less competent than STT3A-containing complexes for cotranslational glycosylation, they have the ability to mediate glycosylation of some nascent sites that are not accessible for STT3A. STT3B-containing complexes also act post-translationally and mediate modification of skipped glycosylation sites in unfolded proteins. Plays a role in ER-associated degradation (ERAD) pathway that mediates ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins by mediating N-glycosylation of unfolded proteins, which are then recognized by the ERAD pathway and targeted for degradation. Mediates glycosylation of the disease variant AMYL-TTR 'Asp-38' of TTR at 'Asn-118', leading to its degradation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: B6dom1 antigen; dolichyl-diphosphooligosaccharide protein glycotransferase; Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B; homolog of yeast STT3; Oligosaccharyl transferase subunit STT3B; Source of immunodominant MHC-associated peptides; Source of immunodominant MHC-associated peptides homolog; STT3, subunit of the oligosaccharyltransferase complex, homolog B; STT3-B; STT3B, subunit of the oligosaccharyltransferase complex (catalytic)
基因别名: 1300006C19Rik; CDG1X; RGD1311563; SIMP; STT3-B; STT3B
UniProt ID: (Human) Q8TCJ2, (Mouse) Q3TDQ1
Entrez Gene ID: (Human) 201595, (Rat) 363160, (Mouse) 68292
