Invitrogen

Sclerostin Polyclonal Antibody

Sclerostin Antibody in Immunohistochemistry (Frozen) (IHC (F))

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Sclerostin Antibody (PA5-46977) in IHC (F)

Immunohistochemical analysis of Sclerostin in immersion fixed frozen sections of mouse embryo (adrenal gland). Samples were incubated in Sclerostin polyclonal antibody (Product # PA5-46977) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).

Sclerostin Antibody in Western Blot (WB)

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产品信息

PA5-46977

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2 µg/mL

免疫组化（冰冻） (IHC (F))

5-15 µg/mL

产品规格

种属反应

Mouse

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

Mouse myeloma cell line NS0-derived recombinant mouse SOST/Sclerostin Gln24-Tyr211

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.2 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 5% trehalose

内含物

No Preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2605802

产品详细信息

In direct ELISAs, less than 2% cross-reactivity with recombinant human SOST is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

靶标信息

Sclerostin, a glycoprotein predominantly secreted by osteocytes, is a member of the Cerberus/DAN family of putative BMP antagonists that functions as an endogenous regulator of the canonical Wnt signaling pathway and an inhibitory regulator of bone homeostasis. Although expressed nearly exclusively by osteocytes, sclerostin can also be found at significant levels elsewhere, such as bone, bone marrow, cartilage, the kidney, and the liver, and has also been shown to be produced by hypertrophic chondrocytes and cementocytes. Like DKK family members DKK-1 and DKK-4, sclerostin plays an important regulatory role in the Wnt/beta-catenin signaling pathway by forming inhibitory complexes with LDL Receptor-related proteins 5 and 6 (LRP5 and LRP6), which are essential components of the Wnt/beta-catenin signaling system. LRP5 and LRP6 are single-pass transmembrane proteins that appear to act as co-receptors for Wnt ligands involved in the Wnt/beta-catenin signaling cascade. Sclerostin has also been shown to interact directly with LRP4 via its extracellular domain to facilitate inhibition of Wnt signaling, and can catabolically promote osteoclast activity by increasing osteocyte expression of RANKL. Sclerostin's critical involvement in the regulation of bone formation and resorption is emphasized by two bone dysplasia disorders, sclerosteosis and van Buchem disease (VBD), caused by rare autosomal recessive mutations that result in progressive bone overgrowth and hypermineralization due to markedly decreased sclerostin levels.

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