Invitrogen

Sclerostin Polyclonal Antibody

Sclerostin Antibody in Western Blot (WB)

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Sclerostin Antibody (PA5-85681) in WB

Sclerostin Polyclonal Antibody detects Sclerostin protein by western blot analysis. HepG2 whole cell extracts and conditioned medium (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Sclerostin Polyclonal Antibody (Product # PA5-85681) diluted at 1:500.

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产品信息

PA5-85681

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein encompassing a sequence within the center region of human Sclerostin.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2.73 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2792820

产品详细信息

Keep as concentrated solution.

Predicted reactivity: Mouse (89%), Rat (92%), Rhesus Monkey (97%), Bovine (94%).

Positive Control: HepG2 conditioned medium.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Sclerostin, a glycoprotein predominantly secreted by osteocytes, is a member of the Cerberus/DAN family of putative BMP antagonists that functions as an endogenous regulator of the canonical Wnt signaling pathway and an inhibitory regulator of bone homeostasis. Although expressed nearly exclusively by osteocytes, sclerostin can also be found at significant levels elsewhere, such as bone, bone marrow, cartilage, the kidney, and the liver, and has also been shown to be produced by hypertrophic chondrocytes and cementocytes. Like DKK family members DKK-1 and DKK-4, sclerostin plays an important regulatory role in the Wnt/beta-catenin signaling pathway by forming inhibitory complexes with LDL Receptor-related proteins 5 and 6 (LRP5 and LRP6), which are essential components of the Wnt/beta-catenin signaling system. LRP5 and LRP6 are single-pass transmembrane proteins that appear to act as co-receptors for Wnt ligands involved in the Wnt/beta-catenin signaling cascade. Sclerostin has also been shown to interact directly with LRP4 via its extracellular domain to facilitate inhibition of Wnt signaling, and can catabolically promote osteoclast activity by increasing osteocyte expression of RANKL. Sclerostin's critical involvement in the regulation of bone formation and resorption is emphasized by two bone dysplasia disorders, sclerosteosis and van Buchem disease (VBD), caused by rare autosomal recessive mutations that result in progressive bone overgrowth and hypermineralization due to markedly decreased sclerostin levels.

仅用于科研。不用于诊断过程。未经明确授权不得转售。