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Invitrogen
Smooth Muscle Myosin Monoclonal Antibody (SMMS-1), eBioscience™
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Smooth Muscle Myosin Antibody (14-6400-82) in IHC (P)
Immunohistochemistry on formalin-fixed paraffin embedded human infiltrating ductal carcinoma, using 5 µg/mL of Mouse IgG1 K Isotype Control Purified (Product # 14-4714-82) (left) or 5 µg/mL Anti-Smooth Muscle Myosin Purified (right) followed by Anti-Mouse IgG HRP, and DAB visualization.Nuclei are counterstained with hematoxylin.
产品信息
14-6400-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Bovine,
Dog,
Cat,
Human,
Rat
已发表种属
Human
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
SMMS-1
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_10714989
产品详细信息
Description: This SMMS-1 monoclonal antibody reacts with both isoforms of the human smooth muscle myosin heavy chain (SM1 and SM2) structural proteins, which are components of the contractile apparatus in smooth muscle cells. Smooth muscle myosin heavy chains are expressed by myoepithelial cells and also serve as the terminal differentiation marker for smooth muscle cells. In normal breast tissue, myoepithelial cells compose the outer layer of the lumen lining. In benign and in situ breast lesions, the intact myoepithelial layer stains with SMMS-1 and the loss of this staining indicates invasive cancer. This SMMS-1 antibody has also been reported to cross-react with bovine, canine, feline, and rat smooth muscle myosin heavy chains.
Applications Reported: This SMMS-1 antibody has been reported for use in western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.
Applications Tested: This SMMS-1 antibody has been tested by western blot on rat heart and immunohistochemistry on formalin-fixed paraffin embedded tissue (with IHC Antigen Retrieval Solution - Low pH (Product # 00-4955-58). This can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be titrated for optimal perfomance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
SM-MHC is a cytoplasmic structural protein, which is a major component of the contractile apparatus in smooth muscle cells. Expression of smooth muscle myosin is developmently regulated, appearing early in smooth muscle development, and is specific for smooth muscle development. Two isoforms of smooth muscle myosin heavy chain have been identified, designated MHC-1 and MHC-2.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: contractile protein; myh11 {ECO:0000312|RGD:3136}; Myosin heavy chain 11; myosin heavy chain 21; myosin heavy chain SMA; Myosin heavy chain, smooth muscle isoform; myosin, heavy chain 11, smooth muscle; myosin, heavy polypeptide 11, smooth muscle; Myosin-11; SMMHC; smooth muscle myosin heavy chain
基因别名: AAT4; FAA4; KIAA0866; MYH11; SMHC; SMMHC
UniProt ID: (Human) P35749
Entrez Gene ID: (Bovine) 530050, (Human) 4629, (Dog) 479836, (Rat) 24582
