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Rockland

Swi6 Polyclonal Antibody

说明书

实验方案

技术支持

货号 600-401-867

CNY 5,930.00

100 µg

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说明书

实验方案

技术支持

抗体检测数据 (2)

Application

图: 1 / 2

Swi6 Antibody (600-401-867) in WB

Western blot analysis is shown using Rocklands Affinity Purified anti-Swi6 antibody to detect endogenous protein present in S. pombe lysate (arrowhead). Comparison to a molecular weight marker (not shown) indicates a band of ~43 kDa corresponding to S. pombe Swi6 protein. ~35µg of lysate was loaded per lane onto a 4-20% gradient gel for SDS-PAGE followed by transfer to 0.45 µm nitrocellulose. The blot was incubated with a 1:1,700 dilution of the antibody at room temperature for 2 h followed by detection using IRDye™800 labeled Goat-a-Rabbi... View More

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Swi6 Antibody — The distribution of Ndc80, Cnp1, and Swi6 proteins are indicated in relation to the observed centromeric anchor and heterochromatin structures. See Kniola et al for additional details.

产品信息

600-401-867

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

酶联免疫吸附实验 (ELISA)

1:5,000-1:30,000

免疫沉淀 (IP)

1:500-1:3,000

产品规格

种属反应

Yeast

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

This anti-Swi6 Antibody was prepared from whole rabbit antiserum produced by repeated immunizations with a synthetic peptide corresponding aa 314-328 of S.pombe Swi6 protein.

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偶联物

Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

内含物

0.01% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

BLAST analysis indicates that cross reactivity with homologues of this protein from other sources is not likely.

靶标信息

Swi6 protein of fission yeast is a functional and structural homolog of HP1 (Heterochromatin Protein 1) of animals and is involved in the formation of heterochromatin structure by binding to centromere, telomere and silent mating-type locus. It is also involved in silencing the genes and sister chromatid cohesion by binding to histone H3 methylated at Lys9 and the cohesin subunit Psc3.

仅用于科研。不用于诊断过程。未经明确授权不得转售。