Invitrogen

Syk Recombinant Rabbit Monoclonal Antibody (PSH0-76)

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (3)
高级验证 (2)

Syk Antibody in Immunocytochemistry (ICC/IF)

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Syk Antibody (MA5-49362) in ICC/IF

Immunofluorescence analysis of Syk was performed using 70% confluent log phase Ramos and Jurkat cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with Syk Rabbit Recombinant Monoclonal Antibody (PSH0-76) (Product # MA5-49362) at 1:100 dilution in 0.1% BSA, incubated at 4°C overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjuga... View More

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Syk Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of Syk was observed in Raji, Daudi, Ramos, U-937, K-562 and THP-1 which are reported to be positive for Syk expression and not in any other cell lines like Jurkat, RD and PANC-1 using Syk Rabbit Recombinant Monoclonal Antibody (PSH0-76) (Product # MA5-49362) in Western Blot. {RE}

产品信息

MA5-49362

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

PSH0-76

抗原

Synthetic peptide within human aa 451-500 / 635.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_3075019

产品详细信息

Sequence Similarities: 100% Mouse.

Tissue Specificity: Widely expressed in hematopoietic cells (at protein level) (PubMed:8163536). Expressed in neutrophils (at protein level) (PubMed:15123770). Within the B-cell compartment, expressed from pro- and pre-B cells to plasma cells (PubMed:8163536).

Positive Control: Raji cell lysate, Daudi cell lysate, SW620 cell lysate, Ramos cell lysate.

Subcellular Location: Cell membrane, Cytoplasm, cytosol.

Predicted band size: 72 kDa.

靶标信息

Spleen tyrosine kinase (SYK), along with ZAP70, are cytoplasmic tyrosine kinases that are viewed as potential druge targets for allergic disorders and autoimmune diseases. SYK may also play a role in cancer. Syk translocates to the plasma membrane upon B cell antigen receptor (BCR) or the high-affinity IgE receptor (FcepsilonRI) triggering, and phosphorylates downstream adaptor proteins, thereby providing docking sites for initiation of subsequent signaling pathways, such as calcium mobilization, cytoskeleton remodeling, or transcription of specific genes. Syk binds to the receptor assemblies through interactions of its pair of SH2 domains with ITAM motives of the receptor, which have been phosphorylated by Src-family kinases. These kinases also help to activate Syk by phosphorylation of its activation loop.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: DKFZp313N1010; EC 2.7.10.2; FLJ25043; FLJ37489; HGNC:11205; kinase Syk; KSYK; p72-Syk; SP1; Spleen tyrosine kinase; syk signaling; tyrosine-kinase syk; Tyrosine-protein kinase SYK

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基因别名: p72-Syk; SYK

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UniProt ID: (Human) P43405

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Entrez Gene ID: (Human) 6850

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功能

protein kinase activity protein serine/threonine kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity integrin binding protein binding ATP binding protein kinase binding

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