Invitrogen

Syk Monoclonal Antibody (SYK-01)

高级验证

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

4个已发表图片

5 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

Syk Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 15

Syk Antibody (MA1-19332) in ICC/IF

Immunofluorescence of SYK was performed using THP-1 wild-type and SYK KO cells that were transfected with a green or a far-red fluorescent dye, respectively. Post-transfection, WT and KO cells were mixed and plated to a 1:1 ratio on coverslips as a mosaic and incubated for 24 hrs. Cells were fixed in 4% PFA (in PBS) or methanol for 15 min; cells were permeabilized with 0.1% Triton X-100 for 10 min at RT and blocked with PBS with 5% BSA, 5% goat serum, and 0.01% Triton X-100 for 30 min. Cells were stained with the SYK monoclonal antibody (P... View More

Syk Antibody in Western Blot, Immunoprecipitation (WB, IP)

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Syk Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Higher expression of Syk was observed specifically in B-lymphocytes (Ramos) in comparison to other hematopoietic cell lines (THP-1, Jurkat) using Anti-Syk Monoclonal Antibody (Product # MA1-19332) in western blot. {RE}

产品信息

MA1-19332

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

5 µg/mL

免疫细胞化学 (ICC/IF)

Assay-dependent

免疫沉淀 (IP)

Assay-dependent

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

SYK-01

抗原

Recombinant fragment (aa 5-360) of human Syk.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

15mM sodium azide

保存条件

4° C, do not freeze

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2197214

产品详细信息

This antibody reacts with protein tyrosine kinase p72Syk (Syk; an intracellular antigen), which is required for the transduction of signals through the B cell antigen receptor (BCR) and the high affinity IgE receptor (FcepsilonRI).

靶标信息

Spleen tyrosine kinase (SYK), along with ZAP70, are cytoplasmic tyrosine kinases that are viewed as potential druge targets for allergic disorders and autoimmune diseases. SYK may also play a role in cancer. Syk translocates to the plasma membrane upon B cell antigen receptor (BCR) or the high-affinity IgE receptor (FcepsilonRI) triggering, and phosphorylates downstream adaptor proteins, thereby providing docking sites for initiation of subsequent signaling pathways, such as calcium mobilization, cytoskeleton remodeling, or transcription of specific genes. Syk binds to the receptor assemblies through interactions of its pair of SH2 domains with ITAM motives of the receptor, which have been phosphorylated by Src-family kinases. These kinases also help to activate Syk by phosphorylation of its activation loop.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Neuronal C-Reactive Protein/FcγRI Positive Feedback Proinflammatory Signaling Contributes to Nerve Injury Induced Neuropathic Pain.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Liu F,Zhang L,Su S,Fang Y,Yin XS,Cui H,Sun J,Xie Y,Ma C

MA1-19332 was used in Immunohistochemistry-immunofluorescence to provide novel insights into the neuroimmune responses after peripheral nerve injury and suggest potential therapeutic targets for neuropathic pain.

Sat Apr 01 00:00:00 EDT 2023

Amyloid-β activates NLRP3 inflammasomes by affecting microglial immunometabolism through the Syk-AMPK pathway.

Aging cell

Jung ES,Suh K,Han J,Kim H,Kang HS,Choi WS,Mook-Jung I

MA1-19332 was used in Western Blot to suggest that repurposing of FA for inhibiting microglial activation of the NLRP3 inflammasome is a potential treatment for AD.

Sun May 01 00:00:00 EDT 2022

Amyloid-β activates NLRP3 inflammasomes by affecting microglial immunometabolism through the Syk-AMPK pathway.

Aging cell

Jung ES,Suh K,Han J,Kim H,Kang HS,Choi WS,Mook-Jung I

MA1-19332 was used in Western Blot to suggest that repurposing of FA for inhibiting microglial activation of the NLRP3 inflammasome is a potential treatment for AD.

Sun May 01 00:00:00 EDT 2022

An Allosteric Shift in CD11c Affinity Activates a Proatherogenic State in Arrested Intermediate Monocytes.

Journal of immunology (Baltimore, Md. : 1950)

Hernandez AA,Foster GA,Soderberg SR,Fernandez A,Reynolds MB,Orser MK,Bailey KA,Rogers JH,Singh GD,Wu H,Passerini AG,Simon SI

MA1-19332 was used in Western Blotting to conclude that CD11c functions as a mechanoregulator that activates an inflammatory state preferentially in a majority of iMo from cardiac patients but not healthy patients.

Sun Nov 15 00:00:00 EST 2020

CARD9 negatively regulates NLRP3-induced IL-1β production on Salmonella infection of macrophages.

Nature communications

Pereira M,Tourlomousis P,Wright J,P Monie T,Bryant CE

MA1-19332 was used in immunoprecipitation and western blot to examine the contribution of the CARD9 domain of interleukin-1beta in Salmonella enterica serovar Typhimurium infection

Tue Sep 27 00:00:00 EDT 2016

CARD9 negatively regulates NLRP3-induced IL-1β production on Salmonella infection of macrophages.

Nature communications

Pereira M,Tourlomousis P,Wright J,P Monie T,Bryant CE

MA1-19332 was used in immunoprecipitation and western blot to examine the contribution of the CARD9 domain of interleukin-1beta in Salmonella enterica serovar Typhimurium infection

Tue Sep 27 00:00:00 EDT 2016

生物信息学

蛋白别名: DKFZp313N1010; EC 2.7.10.2; FLJ25043; FLJ37489; HGNC:11205; kinase Syk; KSYK; p72-Syk; p72Syk; SP1; Spleen tyrosine kinase; syk signaling; tyrosine-kinase syk; Tyrosine-protein kinase SYK

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基因别名: p72-Syk; p72syk; ptk72; SYK; Sykb

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UniProt ID: (Human) P43405, (Rat) Q64725, (Mouse) P48025

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Entrez Gene ID: (Human) 6850, (Rat) 25155, (Mouse) 20963

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