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Invitrogen
Synaptophysin Monoclonal Antibody (EP10), eBioscience™
高级验证
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
图: 1 / 5
Synaptophysin Antibody (14-6525-82) in IHC (P)
Immunohistochemistry of formalin-fixed paraffin embedded human cerebellum using 5 µg/mL of Mouse IgG1 kappa Isotype Control (Product # 14-4714-82) (left) or Anti-Human Synaptophysin Purified (right) followed by Anti-Mouse IgG Biotin and DAB visualization.Nuclei are counterstained with hematoxylin.
产品信息
14-6525-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Hamster,
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
EP10
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_10670280
产品详细信息
Description: This EP10 monoclonal antibody reacts with human Synaptophysin, which is also known as Major Synaptic Vesicle Protein p38. This 38-kDa integral membrane protein is expressed on neuronal presynaptic vesicles in the brain, spinal cord, retina, neuromuscular junctions, and adrenal medulla. Synaptophysin is also expressed in neuroendocrine tumors and neuroblastomas. Synaptophysin has been reported to play a role in synaptic vesicle trafficking.
The EP10 antibody recognizes human and hamster synaptophysin but does not recognize mouse and rat synaptophysin.
Applications Reported: This EP10 antibody has been reported for use in immunoprecipitation, immunoblotting (WB), immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry (ICC).
Applications Tested: This EP10 antibody has been tested by immunohistochemistry on formalin fixed paraffin embedded human cerebellum using citrate buffer antigen retrieval. This antibody can be used at less than or equal to 5 µg/mL. This EP10 antibody has also been tested by western blot on human brain lysate and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Synaptophysin is a calcium-binding and integral membrane glycoprotein present in presynaptic vesicles in almost all neurons. Synaptophysin has four transmembrane domains and it forms a complex with dynamin at high calcium concentrations suggesting an involvement in synaptic vesicle endocytosis. It is also involved in the regulation of short-term and long-term synaptic plasticity. Synaptophysin is currently the most widely used marker for nerve terminals and for differentiating neuroendocrine tumors. Mutations in the gene can result in mental retardation, X-linked 96.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Major synaptic vesicle protein p38; Synaptophysin
基因别名: I79_012252; MRX96; MRXSYP; SYP
UniProt ID: (Human) P08247
Entrez Gene ID: (Human) 6855, (Chinese hamster) 100758282
endocytosis
synaptic vesicle maturation
regulation of neuronal synaptic plasticity
regulation of long-term neuronal synaptic plasticity
regulation of short-term neuronal synaptic plasticity
synaptic vesicle membrane organization
cellular response to organic substance
regulation of opioid receptor signaling pathway
