Invitrogen

Synaptotagmin 1 Polyclonal Antibody

Synaptotagmin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Synaptotagmin 1 Antibody (PA5-18856) in IHC (P)

Immunohistochemistry analysis of Synaptotagmin 1 in human brain, cortex. Samples were incubated with Synaptotagmin 1 polyclonal antibody (Product # PA5-18856) using a dilution of 3.75 µg/mL. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Synaptotagmin 1 Antibody in Western Blot (WB)

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产品信息

PA5-18856

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.3-1 µg/mL

免疫组化（石蜡） (IHC (P))

4-6 µg/mL

产品规格

种属反应

Human

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide sequence (RDLQSAEKEEQEK) corresponding to the internal amino acids of SYT1 (aa 261-274).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Ammonium sulfate precipitation

保存液

TBS, pH 7.3, with 0.5% BSA

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_10982413

产品详细信息

This antibody is predicted to react with bovine, canine, mouse and rat based on sequence homology.

This antibody is tested in Peptide ELISA: antibody detection limit dilution 2,000.

靶标信息

Synaptotagmin 1 is a synaptic vesicle membrane glycoprotein that is widely expressed throughout the CNS and is generally thought to act as the Ca2+ sensor in the regulation of exocytosis and neurotransmitter release. Studies indicate that synaptotagmin’s Ca2+ mediated binding of SNAP25 is essential for the Ca2+ dependent triggering of membrane. It has been demonstrated that discrete residues within the c(2)b binding domain of synaptotagmin 1 independently specify endocytic rate and synaptic vesicle size. Synaptotagmin 1 may have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. Further, Synaptotagmin 1 binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has been reported. Synaptotagmin 1 can also bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2. Diseases associated with the dysfunction of Synaptotagmin 1 include food-borne botulism and infant botulism.

仅用于科研。不用于诊断过程。未经明确授权不得转售。