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Application
FIGURE: 1 / 4
TAF1B Antibody (PA5-104353) in ICC/IF
Immunofluorescent analysis of TAF1B in HepG2. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with TAF1B polyclonal antibody (Product # PA5-104353) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
Product Details
PA5-104353
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human TAF1B(Accession Q53T94), corresponding to amino acid residues E147-E197.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2853663
Product Specific Information
Antibody detects endogenous levels of total TAF1B.
Target Information
Component of RNA polymerase I core factor complex that acts as a GTF2B/TFIIB-like factor and plays a key role in multiple steps during trancription initiation such as preinitiation complex (PIC) assembly and postpolymerase recruitment events in polymerase I (Pol I) transcription. Binds rDNA promoters and plays a role in Pol I recruitment as a component of the SL1/TIF-IB complex and, possibly, directly through its interaction with RRN3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: RNA polymerase I-specific TBP-associated factor 63 kDa; RNA polymerase I-specific TBP-associated factor 68 kDa; SL1, 63kD subunit; TAFI63; TAFI68; TATA box binding protein (TBP)-associated factor, RNA polymerase I, B, 63kD; TATA box binding protein (TBP)-associated factor, RNA polymerase I, B, 63kDa; TATA box binding protein associated factor 1B; TATA box-binding protein-associated factor 1B; TATA box-binding protein-associated factor RNA polymerase I subunit B; TATA-box binding protein associated factor, RNA polymerase I, B; TBP-associated factor 1B; TBP-associated factor RNA polymerase I 68; transcription factor TAFI68; Transcription initiation factor SL1/TIF-IB subunit B
Gene Aliases: 4930408G01Rik; A230108M10Rik; MGC:9349; p63; RAF1B; RAFI63; SL1; TAF1B; TAFI63; TAFI68; Tafi86
UniProt ID: (Human) Q53T94, (Mouse) P97358, (Rat) D3ZYB7
Entrez Gene ID: (Human) 9014, (Mouse) 21340, (Rat) 690450
RNA polymerase I CORE element sequence-specific DNA binding
transcription factor activity, RNA polymerase I CORE element binding transcription factor recruiting
transcription factor activity, sequence-specific DNA binding
protein binding
TBP-class protein binding
metal ion binding
DNA binding
molecular_function
RNA polymerase I transcriptional preinitiation complex assembly at the promoter for the nuclear large rRNA transcript
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription initiation from RNA polymerase I promoter
transcription elongation from RNA polymerase I promoter
termination of RNA polymerase I transcription
positive regulation of gene expression, epigenetic
regulation of transcription from RNA polymerase I promoter
transcription from RNA polymerase I promoter
biological_process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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