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Invitrogen
TCR V beta 9 Monoclonal Antibody (MR10-2), eFluor™ 450, eBioscience™
Application
图: 1 / 1
TCR V beta 9 Antibody (48-5823-80) in Flow
Staining of C57Bl/6 lymph node with Anti-Mouse CD3e APC (Product # 17-0031-82) and 0.25 µg of Mouse IgG1 K Isotype Control eFluor® 450 (Product # 48-4714-82) (left) or 0.25 µg of Anti-Mouse V beta 9 TCR eFluor® 450 (right). Cells in the lymphocyte gate were used for analysis.
产品信息
48-5823-80
产品规格
种属反应
Mouse
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
MR10-2
偶联物
激发/发射光谱
405/445 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_10854882
产品详细信息
Description: This MR10-2 monoclonal antibody reacts with the mouse T-cell receptor (TCR) V beta 9 chain. Composed of an a and β chain, TCR specificity is typically determined by Va, Ja, Vb, Db, and Jb gene rearrangement. The MR10-2 antibody recognizes the V beta 9 chain on T cells from mouse strains with the b haplotype, including BALB/c, CBA/Ca, C3H/He, and C57BL. This gene is deleted in mice bearing the a (e.g., C57BR, C57L, SJL, and SWR) or c (e.g, RIII) haplotypes. T cells expressing V beta 9 are also deleted in mice expressing the minor lymphocyte stimulating (MIs)-1a antigen.
The MR10-2 antibody has been reported to activate V beta 9+ T cells.
Applications Reported: Anti-Mouse V beta 9 TCR eFluor® 450 has been reported for use in flow cytometric analysis.
Applications Tested: Anti-Mouse V beta 9 TCR eFluor® 450 has been tested by flow cytometric analysis of mouse lymph node. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease. Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: TCR V beta9; TCRV beta 9; TCRV beta9; Vb9; Vbeta9
