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Invitrogen
TET2 Recombinant Superclonal Antibody (7HCLC)
高级验证
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
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TET2 Antibody (712150) in ICC/IF
For immunofluorescence analysis, HT-29 cells were fixed and permeabilized for detection of endogenous TET2 using Anti-TET2 Recombinant Rabbit Polyclonal Antibody (Product # 712150, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of TET2 protein (green), Panel b) is stained for nuclei (blue) using ProLong™ Diamond Antifade Mountant with DAPI (Product ... View More
产品信息
712150
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Superclonal
类型
Antibody
克隆号
7HCLC
抗原
Protein corresponding to human TET2 [aa1700-aa2002]
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2784612
产品详细信息
This antibody is predicted to react with Monkey, Pig, Cat.
Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
靶标信息
TET2 is a dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydromethylcytosine (5hmC) and plays a key role in active DNA demethylation. TET2 has a preference for 5-hydroxymethylcytosine in CpG motifs. It mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC) and conversion of 5fC to 5-carboyxlcytosine (5caC). Conversion of 5mC into 5hmC, 5fC, and 5caC probably constitutes the first step in cytonsine demethylation. In addition to TET2’s role in DNA demethylation, it is also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT. Mutations in the genes can result in Polycythemia vera and Myelodysplastic syndrome.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: FLJ20032; Methylcytosine dioxygenase TET2; MGC125715; OTTHUMP00000161869; probable methylcytosine dioxygenase TET2; Protein Ayu17 449; Tet 2; tet oncogene family member 2
基因别名: KIAA1546; MDS; Nbla00191; TET2
UniProt ID: (Human) Q6N021
Entrez Gene ID: (Human) 54790
kidney development
myeloid progenitor cell differentiation
5-methylcytosine catabolic process
protein O-linked glycosylation
cell cycle
post-embryonic development
response to organic cyclic compound
cytosine metabolic process
hemoglobin metabolic process
myeloid cell differentiation
positive regulation of transcription from RNA polymerase II promoter
spleen development
oxidation-reduction process
hematopoietic stem cell homeostasis
liver morphogenesis
DNA demethylation
histone H3-K4 trimethylation
