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TET2 Antibody (MA5-27838) in WB
CRISPR-Cas9 mediated genome editing ofTET2 (as confirmed by next generation sequencing) was achieved by using LentiArray™ Lentiviral sgRNA (Product # A32042, AssayID CRISPR796551_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Fig (a) Western blot analysis of TET2 was performed by loading 30 µg of HeLa wild type (Lane 1), HeLa Cas9 (Lane 2) and HeLa Cas9 cells transduced with TET2 Lentiviral sgRNA (Lane 3) modified whole cell extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Product # NP0322... View More
产品信息
MA5-27838
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
GT649
抗原
Recombinant protein encompassing a sequence within the N-terminus region of human TET2. The exact sequence is proprietary.
偶联物
形式
Liquid
浓度
1.0 mg/mL
纯化类型
Protein G
保存液
PBS
内含物
no preservative
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2735393
产品详细信息
Positive Control: 293T, A431, HeLa, HepG2
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
TET2 is a dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydromethylcytosine (5hmC) and plays a key role in active DNA demethylation. TET2 has a preference for 5-hydroxymethylcytosine in CpG motifs. It mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC) and conversion of 5fC to 5-carboyxlcytosine (5caC). Conversion of 5mC into 5hmC, 5fC, and 5caC probably constitutes the first step in cytonsine demethylation. In addition to TET2’s role in DNA demethylation, it is also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT. Mutations in the genes can result in Polycythemia vera and Myelodysplastic syndrome.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: FLJ20032; Methylcytosine dioxygenase TET2; MGC125715; OTTHUMP00000161869; probable methylcytosine dioxygenase TET2; Protein Ayu17 449; Tet 2; tet oncogene family member 2
基因别名: KIAA1546; MDS; Nbla00191; TET2
UniProt ID: (Human) Q6N021
Entrez Gene ID: (Human) 54790
kidney development
myeloid progenitor cell differentiation
5-methylcytosine catabolic process
protein O-linked glycosylation
cell cycle
post-embryonic development
response to organic cyclic compound
cytosine metabolic process
hemoglobin metabolic process
myeloid cell differentiation
positive regulation of transcription from RNA polymerase II promoter
spleen development
oxidation-reduction process
hematopoietic stem cell homeostasis
liver morphogenesis
DNA demethylation
histone H3-K4 trimethylation
