Invitrogen

TGF beta-3 Polyclonal Antibody

TGF beta-3 Antibody in Western Blot (WB)

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TGF beta-3 Antibody (PA5-97064) in WB

Western blot analysis of TGF beta-3 in extracts of various cell lines. Samples were incubated with TGF beta-3 Polyclonal antibody (Product # PA5-97064) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

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产品信息

PA5-97064

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 25-300 of human TGFB3 (NP_0032301)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2.11 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2808866

产品详细信息

Immunogen sequence: STCTTLDFGH IKKKRVEAIR GQILSKLRLT SPPEPTVMTH VPYQVLALYN STRELLEEMH GEREEGCTQE NTESEYYAKE IHKFDMIQGL AEHNELAVCP KGITSKVFRF NVSSVEKNRT NLFRAEFRVL RVPNPSSKRN EQRIELFQIL RPDEHIAKQR YIGGKNLPTR GTAEWLSFDV TDTVREWLLR RESNLGLEIS IHCPCHTFQP NGDILENIHE VMEIKFKGVD NEDDHGRGDL GRLKKQKDHH NPHLILMMIP PHRLDNPGQG GQRKKR; Positive Samples: BT-474, K-937, Mouse lung, Mouse skeletal muscle, Mouse heart, Rat lung, Rat heart

靶标信息

Transforming growth factor beta 3 (TGF beta 3) is the third member of the transforming growth factor family of cytokines, which also includes TGF beta 1 and beta 2. These cytokines are secreted in precursor form consisting of a bioactive C-terminal domain attached to an N-terminal domain known as latency associated protein (LAP). Cleavage of LAP results in the mature protein, which functions as a disulfide-linked homodimer. As with all members of the family, TGF beta 3 is highly conserved across species, with mouse and human TGF beta 3 demonstrating 100% sequence homology and cross-species activity.The members of this family can be expressed by most cell types and exert pleiotropic effects, which include the suppression of B- and T-cell effector activity, mediation of tissue healing, and suppression of tumor proliferation. The promotion of CD4+CD25+ T-cell expansion is a newly discovered function of the TGF beta cytokines, and indicates an important role in the protection against autoimmunity.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: FLJ16571; H-TGF-b-3; prepro-transforming growth factor beta-3; tgf beta 3; TGF-beta-3; Transforming growth factor; transforming growth factor beta-3; Transforming growth factor beta-3 proprotein; transforming growth factor, beta 3

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基因别名: ARVD; ARVD1; LDS5; RNHF; TGF-B3; TGF-beta3; Tgfb-3; TGFB3

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UniProt ID: (Human) P10600, (Rat) Q07258

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Entrez Gene ID: (Human) 7043, (Rat) 25717, (Mouse) 21809

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