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Invitrogen
THRB Polyclonal Antibody
高级验证
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
图: 1 / 4
THRB Antibody (PA5-29684) in ICC/IF
Immunofluorescence analysis of THRB was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with THRB Polyclonal Antibody (Product # PA5-29684) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 conjugate (Product # A3279... View More
产品信息
PA5-29684
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fragment corresponding to a region within amino acids 1 and 199 of Human Thyroid Hormone Receptor beta
偶联物
形式
Liquid
浓度
0.76 mg/mL
纯化类型
Antigen affinity chromatography
保存液
0.1M tris glycine, pH 7, with 10% glycerol
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2493188
产品详细信息
Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji.
Predicted reactivity: Mouse (93%), Rat (92%), Sheep (90%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
Thyroid hormone receptors (TR), also known as c-erbA-2, are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences called hormone response elements following activation by the appropriate hormone. Thyroid hormones, through their interaction with TR, effect metabolic processes, growth and development in many tissues by regulating the expression of genes for growth hormone, malic enzyme and several hepatic proteins. There are two forms of TR; TR alpha (on chromosome 17) and TR beta (on chromosome 3). Each of these isoforms also has two isoforms; TR alpha-1 and TRv alpha-2, and TR beta-1 and TR beta-2 respectively. TR alpha-1 and 2 are identical through amino acid 370 where their sequences diverge. TRv alpha-2, which does not bind T3 and is a strong negative regulator of the functional TR, is 80 amino acids longer than TR alpha-1. The corresponding gene for the thyroid hormone receptor beta-1 is NR1A2.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: c-erbA-2; c-erbA-beta; ERBA 2; ERBA BETA; ERBA-BETA; MGC126109; MGC126110; Nuclear receptor subfamily 1 group A member 2; oncogene ERBA2; thyroid hormone nuclear receptor beta variant 1; Thyroid hormone receptor beta; thyroid hormone receptor beta 3; thyroid hormone receptor beta2delta; thyroid hormone receptor, beta (avian erythroblastic leukemia viral (v-erb-a) oncogene homolog 2); thyroid hormone receptor, beta (erythroblastic leukemia viral (v-erb-a) oncogene homolog 2, avian); TR beta; TR beta 1; TR-B1C-ERBA-2; TRbeta2Delta
基因别名: C-ERBA-2; C-ERBA-BETA; c-erbAbeta; ERBA2; GRTH; NR1A2; PRTH; RATT3REC; T3R[b]; T3Rbeta; T3rec; THR1; THRB; THRB1; THRB2; TRbeta
UniProt ID: (Human) P10828, (Mouse) P37242
Entrez Gene ID: (Human) 7068, (Mouse) 21834, (Rat) 24831
DNA binding
transcription factor activity, sequence-specific DNA binding
steroid hormone receptor activity
transcription corepressor activity
thyroid hormone receptor activity
protein binding
zinc ion binding
enzyme binding
chromatin DNA binding
sequence-specific DNA binding
thyroid hormone binding
double-stranded DNA binding
protein homodimerization activity
metal ion binding
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
C4 zinc finger nuclear receptor
DNA-binding transcription factor
zinc finger transcription factor
gene-specific transcriptional regulator
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
transcription initiation from RNA polymerase II promoter
sensory perception of sound
negative regulation of female receptivity
regulation of heart contraction
female courtship behavior
organ morphogenesis
intracellular receptor signaling pathway
steroid hormone mediated signaling pathway
positive regulation of transcription from RNA polymerase II promoter
Type I pneumocyte differentiation
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
positive regulation of cell proliferation
regulation of lipid metabolic process
positive regulation of chondrocyte differentiation
positive regulation of ossification
negative regulation of transcription, DNA-templated
regulation of cholesterol metabolic process
regulation of triglyceride metabolic process
embryonic organ development
